ModifiSite™ Pyrophosphatase Inhibitor Assay Kit

CAT#: ITS-1125-WB28
Product Type: Assay Kit
Species: All Species
Target: Inorganic pyrophosphatase (PPase, E.C. 3.6.1.1) and related pyrophosphate-hydrolyzing enzymatic complexes.
Brand: ModifiSite
Development Stage: Research-Grade Protein-Based Assay Development
Short Description
Quantifies inorganic pyrophosphatase activity and screens inhibitors using a colorimetric (620 nm) method.
Brand
ModifiSite
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ Pyrophosphatase Inhibitor Assay Kit offers a safe, non-radioactive platform for high-throughput screening of enzyme inhibitors. This homogeneous "mix-incubate-measure" system utilizes a proprietary chromogenic reagent to quantify inorganic phosphate release, providing a robust and automation-ready solution for drug discovery and lead optimization in nucleic acid and protein synthesis research.
Features
Advanced Sensitivity: Detects nanomolar changes in orthophosphate release, ensuring reliable evaluation of low-potency inhibitors in diverse biological matrices.
Streamlined Homogeneous Format: Utilizes a "mix-incubate-measure" workflow at room temperature, eliminating the need for complex wash steps or incubators.
HTS-Compatible Design: Optimized for 96/384-well platforms, delivering a robust Z' factor of 0.59 for automated large-scale compound library screening.
Exceptional Reagent Specificity: Features a non-radioactive colorimetric system with minimal cross-reactivity, providing clear readouts even in complex protein-rich samples.
Process Relevance
Monitoring inorganic pyrophosphatase activity to accelerate lead optimization and validate inhibitory potency during enzymatic drug development cycles.
Application Stage
Lead identification, high-throughput compound screening, and preclinical evaluation of antimicrobial or metabolic drug candidates.
Applications
Facilitating allosteric inhibitor characterization, IC50 determination, and modulator evaluation across yeast, bacterial, and mammalian pyrophosphatases for antimicrobial and metabolic disease research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Inorganic pyrophosphatase (PPase, E.C. 3.6.1.1) and related pyrophosphate-hydrolyzing enzymatic complexes.
Detection Method
Absorbance at 620 nm
Sample Type
Chemical compounds
Research Areas
Antimicrobial drug discovery, parasitology, oncology therapeutics, bioenergetics, etc.
Size
100 tests
Data Output Type
Quantitative colorimetric enzyme activity measurement (absorbance at 620 nm) via endpoint or kinetic analysis.
Assay Time
70 min
Species
All Species
Storage
Pyrophosphate at −20 °C, all other components at 4 °C; upon receipt. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles for the Pyrophosphate. During use, minimize the time components are exposed to room temperature and return them promptly to their respective storage temperatures after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
Inorganic pyrophosphatase is a critical metabolic "cleaner" that prevents the cytotoxic accumulation of pyrophosphate, a byproduct of polymerization. By driving the hydrolysis of PPi to 2Pi, it ensures the thermodynamic favorability of essential biosynthetic reactions.
Pathways
This enzyme facilitates nucleic acid synthesis, protein translation, and fatty acid β-oxidation by shifting chemical equilibria. It regulates phosphate homeostasis and couples energy release to drive unfavorable transformations in central metabolism.
For Research Use Only | Not For Clinical Use
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