Short Description
Quantifies inorganic pyrophosphatase activity and screens inhibitors using a colorimetric (620 nm) method.
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ Pyrophosphatase Inhibitor Assay Kit offers a safe, non-radioactive platform for high-throughput screening of enzyme inhibitors. This homogeneous "mix-incubate-measure" system utilizes a proprietary chromogenic reagent to quantify inorganic phosphate release, providing a robust and automation-ready solution for drug discovery and lead optimization in nucleic acid and protein synthesis research.
Features
Advanced Sensitivity: Detects nanomolar changes in orthophosphate release, ensuring reliable evaluation of low-potency inhibitors in diverse biological matrices.
Streamlined Homogeneous Format: Utilizes a "mix-incubate-measure" workflow at room temperature, eliminating the need for complex wash steps or incubators.
HTS-Compatible Design: Optimized for 96/384-well platforms, delivering a robust Z' factor of 0.59 for automated large-scale compound library screening.
Exceptional Reagent Specificity: Features a non-radioactive colorimetric system with minimal cross-reactivity, providing clear readouts even in complex protein-rich samples.
Process Relevance
Monitoring inorganic pyrophosphatase activity to accelerate lead optimization and validate inhibitory potency during enzymatic drug development cycles.
Application Stage
Lead identification, high-throughput compound screening, and preclinical evaluation of antimicrobial or metabolic drug candidates.
Applications
Facilitating allosteric inhibitor characterization, IC50 determination, and modulator evaluation across yeast, bacterial, and mammalian pyrophosphatases for antimicrobial and metabolic disease research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Inorganic pyrophosphatase (PPase, E.C. 3.6.1.1) and related pyrophosphate-hydrolyzing enzymatic complexes.
Detection Method
Absorbance at 620 nm
Sample Type
Chemical compounds
Research Areas
Antimicrobial drug discovery, parasitology, oncology therapeutics, bioenergetics, etc.
Datasheet