ModifiSite™ Urease Assay Kit

CAT#: ITS-1125-HM145
Product Type: Assay Kit
Species: All Species
Brand: ModifiSite
Development Stage: Research-Grade Protein & Enzyme Assay Development
Short Description
Measures urease activity via ammonia production.
Brand
ModifiSite
Development Stage
Research-Grade Protein & Enzyme Assay Development
Description
The ModifiSite™ Urease Assay Kit utilizes the Berthelot reaction to validate urease inhibitors for H. pylori eradication and agricultural efficiency. This assay measures ammonia production, supporting the development of drugs for gastric ulcers and urease inhibitors for fertilizer stability. It is a key tool for screening compounds that modulation nitrogen metabolism in bacteria and plants.
Features
Berthelot reaction; Validates urease inhibitors for H. pylori eradication and agricultural efficiency.
Process Relevance
Infectious Disease (H. pylori)
Application Stage
Hit-to-Lead Screening
Applications
H. pylori infection research and soil/fertilizer analysis.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 670 nm
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium, environment samples
Research Areas
Infectious Disease (H. pylori); Agriculture
Size
100 tests
Data Output Type
Kinetic (Enzyme Activity)
Assay Time
Results in 40 min
LOD
0.003 U/L
Species
All Species
Storage
Store at 4 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid freezing unless otherwise specified. During use, minimize prolonged exposure to room temperature and return components promptly to 2-8 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Urease hydrolyzes urea into ammonia and carbon dioxide. It is a virulence factor for H. pylori and is used agriculturally to manage nitrogen availability from urea fertilizers.
For Research Use Only | Not For Clinical Use
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