ModifiSite™ Urokinase Assay Kit

CAT#: ITS-1125-HM104
Product Type: Assay Kit
Species: All Species
Brand: ModifiSite
Development Stage: Research-Grade Protein & Enzyme Assay Development
Short Description
Measures urokinase-type plasminogen activator activity.
Brand
ModifiSite
Development Stage
Research-Grade Protein & Enzyme Assay Development
Description
The ModifiSite™ Urokinase Assay Kit employs chromogenic substrate cleavage to screen for inhibitors of uPA-mediated metastasis and invasion. This assay validates drugs targeting the plasminogen activation system, which is critical for tissue remodeling and cancer progression. It supports the discovery of anti-metastatic agents by quantifying the activity of this key extracellular protease.
Features
Chromogenic substrate cleavage; Screening inhibitors of uPA-mediated metastasis and invasion.
Process Relevance
Metastasis & Invasion Research
Application Stage
Lead Optimization
Applications
Cancer metastasis and tissue remodeling studies.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Fluorescence Detection (Ex 380 nm / Em 450 nm)
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Oncology (Metastasis)
Size
100 tests
Data Output Type
Kinetic (Enzyme Activity)
Assay Time
Results in 15min
LOD
0.04 U/L
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Urokinase-type Plasminogen Activator (uPA) converts plasminogen to plasmin degrading the extracellular matrix. Overexpression is associated with tumor invasion and metastasis serving as a prognostic marker.
For Research Use Only | Not For Clinical Use
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