Monkey CXCL9 ELISA Kit, Lot 21AO-778 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Monkey CXCL9 in Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate.

This ELISA kit is a solid phase ELISA designed for quantitative determination of Chemokine C-X-C-Motif Ligand 9 (CXCL9).

ELISA

The supplier is only responsible for the kit itself, but not for the samples consumed during the assay.
Please predict the concentration before assaying.
If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits, some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
Fresh samples without long time storage are recommended for the test.

It is recommended that all standards, controls and samples be run in duplicate.
The coefficient of determination of the standard curve should be higher or equal 0.95 and the highest O.D. should be more than 1.0.
Microtiter plates should be allowed - come - room temperature before opening the foil bags.
Samples should be collected in pyrogen/endotoxin-free tubes.
Samples should be frozen if not analyzed shortly after collection.
When possible, avoid use of badly hemolyzed or lipemic serum.
Read absorbance immediately after adding the stop solution.
Because TMB is light sensitive, avoid prolonged exposure - light. Also avoid contact between TMB and metal, otherwise color may develop.

CXCL9

Monkey

Colorimetric

Competition ELISA

Quantitative

Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Microtiter plate (96 wells stripwell) - 1
Enzyme conjugate - 1 vial
Standard A - 1 vial
Standard B - 1 vial
Standard C - 1 vial
Standard D - 1 vial
Standard E - 1 vial
Standard F - 1 vial
Substrate A - 1 vial
Substrate B - 1 vial
Stop solution - 1 vial
Wash solution - 1 vial
Balance solution - 1 vial
Instruction manual - 1

Precision pipettors and disposable tips to deliver 10-1000 μL. A multi-channel pipette is desirable for large assays.
100 mL and 1 L graduated cylinders.
Distilled or deionized water.
Tubes to prepare sample dilutions.
Absorbent paper.
Microplate reader capable of measuring absorbance at 450 nm.
Centrifuge capable of 3000 x g.
Microplate washer or washing bottle.
Incubator (37 °C).
Data analysis and graphing software.

0.1 ng/mL

100 μL

1.5 h

Pre-coated

Samples - Please predict the concentration before assaying. If concentrations are unknown or not within the detection range, a preliminary experiment is recommended to determine the optimal dilution.
Wash solution - Dilute 10mL of wash solution concentrate (100×) with 990mL of deionized or distilled water to prepare 1000mL of wash solution (1×).

1.Secure the desired number of coated wells in the holder then add 50 µL of Standards or Samples to the appropriate well of the antibody pre-coated Microtiter Plate.
2.Add 100 µL of Conjugate to each well. Cover and incubate for 1 hour at 37 °C.
3.Wash the Microtiter Plate using one of the specified methods.
4.Add 50 µL Substrate A to each well.
5.Add 50 µL Substrate B to each well. Cover and incubate for 15 minutes at 20-25 °C. (avoid sunlight)
6.Add 50 µL of Stop Solution to each well. Mix well.
7.Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader immediately.

TMB is non-carcinogenic but it is hazardous in case of skin contact, eye contact, ingestion and inhalation. In case of contact, rinse affected area with plenty of water.
The stop solution provided with this kit is an acid solution. Wear protective gloves, clothing, and face protection.
Avoid generation of aerosols.
All blood components and biological materials should be handled as potentially hazardous. Decontaminate and dispose specimens and all potentially contaminated materials as they could contain infectious agents. The preferred method of decontamination is autoclaving for a minimum of 1 hour a 121.5°C.

Influenced by the factors including cell viability, cell number and also sampling time, samples from cell culture supernatant may not be detected by the kit.
Fresh samples without long time storage are recommended for the test.
The coefficient of determination of the standard curve should be higher or equal 0.95 and the highest O.D. should be more than 1.0.
Samples should be collected in pyrogen/endotoxin-free tubes.
Read absorbance immediately after adding the stop solution.
Because TMB is light sensitive, avoid prolonged exposure to light. Also avoid contact between TMB and metal, otherwise color may develop.

4 °C

6 months

For Research Use only

Monokine Induced By Interferon Gamma (MIg)

CMK; Humig; MIG; SCYB9; crg-10; BB139920; Mig; MuMIG; Scyb9; C-X-C motif chemokine ligand 9; chemokine (C-X-C motif) ligand 9; CXCL9; Cxcl9