Mouse CD74 ELISA Kit, Lot 21AO-1225 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P1136
Product Type: ELISA Kit
Target: CD74
Short Description
The kit is designed for in vitro quantitative measurement of Mouse CD74 in Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate.
Description
Mouse CD74 ELISA Kit.
Applications
ELISA
Application Notes
Optimal working dilution should be determined by the investigator.
Target
CD74
Reactivity
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Specificity
This ELISA antibody pair recognizes Mouse CD74.
Components
Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate
Material not included
Distilled or deionized water
Precision pipettes to deliver 2 μL to 1 μL volumes
Adjustable 1-25 μL pipettes for reagent preparation
100 μL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Note
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Restrictions
For Research Use only
Alternative Name
Cluster of Differentiation 74
Synonyms
DHLAG; HLADG; II; Ia-GAMMA; CLIP; Ii; INVG34; Iclp-1; iclp1; wu:fb11f09; wu:fb12a01; wu:fb13d06; wu:fk94e07; CD74 molecule; CD74 antigen (invariant polypeptide of major histocompatibility complex; class II antigen-associated); CD74 molecule; major histocompatibility complex; class II invariant chain a; CD74; Cd74; cd74a
Background
CD74, HLA class II histocompatibility antigen gamma chain, CLIP, DHLAG, HLADG, Ii, II, MHC HLA-DR gamma chain, p33, Ia-gamma, INVG34, CD74 antigen, HLA-DR antigens-associated invariant chain, HLA-DR-gamma, Ia antigen-associated invariant chain, Ia-associated invariant chain
Gene ID
16149
UniProt
P04441
Pathways
Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints
Protocol
1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 μL of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 μL of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 μL of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 μL of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 μL of Stop Solution to each well.
11.Read at 450 nm immediately.
For Research Use Only | Not For Clinical Use
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