NCI-H508 In Vitro BrdU Assay (Cell Proliferation)

CAT#: ITS-1122-YF539
Target Cell Organism: Human
Target Cell Alternative Name: H508
Target Cell Name: NCI-H508
Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide NCI-H508-based In Vitro BrdU Assay (Cell Proliferation) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
NCI-H508
Target Cell Organism
Human
Target Cell Background
NCI-H508 [H508] is a cell line derived from metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil. NCI-H508 [H508] is from the cecum of a White, 55-year-old, male patient with colorectal adenocarcinoma. This cell line was deposited by AF Gazdar.
Target Cell Alternative Name
H508
Related Diseases
Colorectal Adenocarcinoma; Colon Cancer
Research Area
Oncology
Assay Name
In Vitro BrdU Assay (Cell Proliferation)
Short Description
NCI-H508-cell based In Vitro BrdU Assay (Cell Proliferation)
Assay Description
5-Bromo-2'-deoxyuridine (BrdU) assay is another methodological approach used to determine cell proliferation. BrdU incorporated into synthesized DNA can be determined using BrdU-specific monoclonal antibody. A secondary antibody is used to generate fluorescent sig nal on DNA synthesis.
Assay Alternative Names
5-Bromo-2'-deoxyuridine (BrdU) Assay
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Proliferation Assay
Assay MOA
Cell-based Assays Based on DNA Synthesis
For Research Use Only | Not For Clinical Use
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