NCI-H508 In Vitro Post-translational Modifications Assay

This assay is to provide NCI-H508-based In Vitro Post-translational Modifications Assay to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

NCI-H508

Human

NCI-H508 [H508] is a cell line derived from metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil. NCI-H508 [H508] is from the cecum of a White, 55-year-old, male patient with colorectal adenocarcinoma. This cell line was deposited by AF Gazdar.

H508

Colorectal Adenocarcinoma; Colon Cancer

Oncology

In Vitro Post-translational Modifications Assay

NCI-H508-cell based In Vitro Post-translational Modifications Assay

Immunoassays and mass spectrometry techniques are considered ideal techniques for the detection of post-translational modification. Both the genetic and epigenetic modifications have contributed to the initiation and progression of cancer.

Gene and Protein Expression Assays

Following transcription, primary transcript RNA is converted into mature RNA by post-transcriptional modifications. Post-transcriptional modification includes three major steps namely the addition of a 5'cap, addition of a 3'polyadenylation tail and RNA splicing. Stability of RNA has been considered as very important for gene expression and proteins at the post-transcriptional level.