NCI-H522 In Vitro Calcein AM Cytotoxic Assay (Cell Viability)

This assay is to provide NCI-H522-based In Vitro Calcein AM Cytotoxic Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

NCI-H522

Human

This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lung cancer obtained from a patient prior to therapy.­

H522

Lung Cancer

Oncology

In Vitro Calcein AM Cytotoxic Assay (Cell Viability)

Calcein AM is another non-fluorescent cell permeable dye, which is converted to green-fluorescent calcein by viable intracellular esterases. Calcein violet AM, calcein red-orange, Oregon green 488 and 5-CFDA-AM are other examples of dyes used to monitor cell viability based on esterase activity in viable cells.

Cell Viability/Cytotoxicity and Antiproliferative Assays

Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.

Cell Viability Assay

Cell-based Viability Assays Based on Cellular Enzymes and Proteins