OncoIHC™ AXIN2 Ready-To-Use IHC Kit

Achieve sensitive and specific AXIN2 staining with this IHC kit, which offers a complete solution with carefully formulated reagents for optimal performance and accurate results.

Axis inhibition protein 2 (AXIN2), also known as Coductin or Axil, is a multidomain scaffold protein that negatively regulates Wnt signaling. AXIN2 can directly interact with beta-catenin and GSK3B. AXIN2 has several phosphorylation sites and can undergo poly(ADP-ribosyl)ation by tankyrase TNKS and TNKS2. Poly(ADP-ribosyl)lated AXIN2 is then ubiquitinated by RNF146, leading to its degradation and activation of Wnt signaling. AXIN2 is localized in the cytoplasm, and mutations in AXIN2 are involved in colorectal cancer.

Ready to use
High Sensitivity Streptavidin (HSS) increases detection sensitivity
Suitable for tissue sections & cytospin preparations
Pre-diluted reagents minimize hands-on time
Optimizes mouse, rabbit, rat, goat, or sheep IgG primary antibody staining

IHC

AXIN2

Human

Immunohistochemistry

FFPE tissue

Primary Antibody
Secondary Antibody
Antigen Unmasking Buffer
Washing Buffer
Blocking Buffer
Color Development Reagent 1
Color Development Reagent 77
Signal Amplification Reagent
Contrast Staining Agent
Preservation Matrix

To ensure the preservation of their functional properties, all reagents within this kit must be maintained at a temperature between 2°C and 8°C. Under these storage conditions, the kit will retain its effectiveness for a period of 6 to 12 months following receipt.

AXIL; axin 2; Axin like protein; AXIN2; Axis inhibition protein 2; Conductin

Creative Biolabs control slides are prepared using top quality tissues that have been processed, embedded and sectioned following standard operating procedure. We provide the following antigen (tissue) controls:
- Positive control: a section from a tissue known to express the protein of interest.
- Negative control: a section from a tissue known not to express the target antigen.
- Endogenous tissue background control: a section from the tissue that doesn't have a primary antibody applied.

Creative Biolabs also offers biospecimen including formalin fixed paraffin embedded (FFPE) tissue blocks, fresh or snap frozen tissue blocks, tissue microarray (TMA) slides. Please contact us to request a customized price quotation.

Tissue Fixative Solution (Specified, depends on antigen type and tissue source)
Tissue Processing Kit (Includes specialized media for tissue collection, isolation, and dissociation)
Tissue Embedding Media
Tissue Staining Kit (Optional)