For the immunohistochemical staining of CD3, this complete kit requires minimal preparation. All reagents are included, from the start of the antigen retreival process, to the final step of applying mounting media. Following the protocol, allows for the quick generation of high quality IHC data.
Description
CD3 is a protein complex associated with the T cell receptor (TCR). The TCR/CD3 complex includes TCR alpha/beta or gamma/delta heterodimers coexpressed with invariant CD3 subunits. The TCR recognizes antigens bound to MHC molecules, transmitting signals to the CD3 complex and facilitating T-cell activation. CD3 is a pan-T cell marker, used for detecting normal and neoplastic T cells.
Features
Ready to use High Sensitivity Streptavidin (HSS) increases detection sensitivity Suitable for tissue sections & cytospin preparations Pre-diluted reagents minimize hands-on time Optimizes mouse, rabbit, rat, goat, or sheep IgG primary antibody staining
Applications
IHC
Target
CD3
Reactivity
Human
Analytical Method
Immunohistochemistry
Sample Type
FFPE tissue
Components
Primary Antibody Secondary Antibody Antigen Unmasking Buffer Washing Buffer Blocking Buffer Color Development Reagent 1 Color Development Reagent 8 Signal Amplification Reagent Contrast Staining Agent Preservation Matrix
Storage
To ensure the preservation of their functional properties, all reagents within this kit must be maintained at a temperature between 2°C and 8°C. Under these storage conditions, the kit will retain its effectiveness for a period of 6 to 12 months following receipt.
Synonyms
CD3; CD3 epsilon; CD3E; CD3-epsilon; T3E; TCRE
IHC Control Slides
Creative Biolabs control slides are prepared using top quality tissues that have been processed, embedded and sectioned following standard operating procedure. We provide the following antigen (tissue) controls: - Positive control: a section from a tissue known to express the protein of interest. - Negative control: a section from a tissue known not to express the target antigen. - Endogenous tissue background control: a section from the tissue that doesn't have a primary antibody applied.
Tissue Samples
Creative Biolabs also offers biospecimen including formalin fixed paraffin embedded (FFPE) tissue blocks, fresh or snap frozen tissue blocks, tissue microarray (TMA) slides. Please contact us to request a customized price quotation.
Tissue Processing Kit
Tissue Fixative Solution (Specified, depends on antigen type and tissue source) Tissue Processing Kit (Includes specialized media for tissue collection, isolation, and dissociation) Tissue Embedding Media Tissue Staining Kit (Optional)