OncoIHC™ HLA-E Ready-To-Use IHC Kit

Facilitate HLA-E detection with this ready-to-use IHC solution, streamlining procedures from antigen retrieval through slide preparation.

Human major histocompatibility complex (MHC) antigens, also known as human leukocyte antigens (HLA), are encoded by genes on the short arm of chromosome 6 (6p21.3). The class I molecules, including HLA-A, B, and C, consist of a heavy (alpha) chain encoded by a class I gene and a β2-microglobulin chain. These molecules are involved in presenting foreign antigens to the immune system. HLA-E is a non-classical MHC class I molecule that is often overexpressed in tumors, transplanted tissues, and virus-infected cells. HLA-E interacts with the CD94/NKG2A, -B, and -C receptors on NK and T cells, functioning as an immune suppressor. This immune suppression enables tumors to evade immune surveillance.

Ready to use
High Sensitivity Streptavidin (HSS) increases detection sensitivity
Suitable for tissue sections & cytospin preparations
Pre-diluted reagents minimize hands-on time
Optimizes mouse, rabbit, rat, goat, or sheep IgG primary antibody staining

IHC

HLA-E

Human

Immunohistochemistry

FFPE tissue

Primary Antibody
Secondary Antibody
Antigen Unmasking Buffer
Washing Buffer
Blocking Buffer
Color Development Reagent 1
Color Development Reagent 291
Signal Amplification Reagent
Contrast Staining Agent
Preservation Matrix

To ensure the preservation of their functional properties, all reagents within this kit must be maintained at a temperature between 2°C and 8°C. Under these storage conditions, the kit will retain its effectiveness for a period of 6 to 12 months following receipt.

EA1.2; EA2.1; HLA 6.2; HLAE; HLA-E; QA1

Creative Biolabs control slides are prepared using top quality tissues that have been processed, embedded and sectioned following standard operating procedure. We provide the following antigen (tissue) controls:
- Positive control: a section from a tissue known to express the protein of interest.
- Negative control: a section from a tissue known not to express the target antigen.
- Endogenous tissue background control: a section from the tissue that doesn't have a primary antibody applied.

Creative Biolabs also offers biospecimen including formalin fixed paraffin embedded (FFPE) tissue blocks, fresh or snap frozen tissue blocks, tissue microarray (TMA) slides. Please contact us to request a customized price quotation.

Tissue Fixative Solution (Specified, depends on antigen type and tissue source)
Tissue Processing Kit (Includes specialized media for tissue collection, isolation, and dissociation)
Tissue Embedding Media
Tissue Staining Kit (Optional)