Development Stage: Research-Grade Metabolic Assay Development
Short Description
Quantifies DNA concentration using a fluorimetric method.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSigh™ DNA Quantification Kit offers a highly sensitive fluorometric method for quantifying double-stranded DNA in biological samples and purified preparations. Utilizing a specific fluorescent dye that exhibits minimal interference from RNA and proteins, the system provides precise measurements across a broad dynamic range. This robust tool is ideal for researchers monitoring cell proliferation, quantification for molecular cloning, and normalization in fundamental genomic and cellular studies.
Features
Features a dsDNA-specific fluorescent dye for rapid, ultra-sensitive DNA quantification, providing high accuracy for genomic research and sample preparation with minimal RNA interference.
Process Relevance
Quantifying dsDNA concentration to verify sample yield and optimize downstream molecular biology protocols.
Application Stage
Molecular biology research and genomic DNA quantification.
Applications
Quantify double-stranded DNA in purified samples and cell lysates for genomic analysis and normalization of molecular biology experiments.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Genomics; Molecular Biology; Nucleic Acid Research
Size
250 tests
Data Output Type
Quantitative concentration measurement
Assay Time
5 min
LOD
LOD: 100 ng/mL
Species
All Species
Storage
Store at 4 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
The precise quantification of double-stranded DNA is essential for various applications, including genomics, cloning, and monitoring cell proliferation. Using dyes that specifically bind to dsDNA provides a more accurate measurement than traditional UV absorbance methods.
Pathways
Antioxidants function as the primary defense against reactive oxygen species (ROS), acting upstream to prevent oxidative damage to lipids, proteins, and DNA. They are critical components of the cellular redox homeostasis network.