OXPHOSight™ Aconitase Assay Kit

CAT#: ITS-1125-HM105
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Quantifies aconitase activity in mitochondria or tissue.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Aconitase Assay Kit measures the conversion of isocitrate, serving as a sensitive marker for oxidative damage to iron-sulfur clusters in mitochondria. This assay is used to evaluate the impact of oxidative stress and superoxide on mitochondrial function. It supports research into neurodegenerative diseases and toxicology, helping to assess the protective effects of antioxidants on enzyme stability.
Features
Measures conversion of isocitrate; Sensitive marker for oxidative damage to iron-sulfur clusters in mitochondria.
Process Relevance
Mitochondrial Oxidative Damage
Application Stage
Basic Research
Applications
Oxidative stress marker in mitochondria.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565 nm
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Oxidative Stress; Mitochondrial Biology
Size
100 tests
Data Output Type
Kinetic (Enzyme Activity)
Assay Time
Results in 30 min
LOD
0.5 U/L
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Aconitase contains an iron-sulfur cluster highly sensitive to oxidative stress. Loss of aconitase activity is a recognized marker of mitochondrial oxidative damage and superoxide generation.
For Research Use Only | Not For Clinical Use
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