Short Description
Quantifies aconitase activity in mitochondria or tissue.
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Aconitase Assay Kit measures the conversion of isocitrate, serving as a sensitive marker for oxidative damage to iron-sulfur clusters in mitochondria. This assay is used to evaluate the impact of oxidative stress and superoxide on mitochondrial function. It supports research into neurodegenerative diseases and toxicology, helping to assess the protective effects of antioxidants on enzyme stability.
Features
Measures conversion of isocitrate; Sensitive marker for oxidative damage to iron-sulfur clusters in mitochondria.
Process Relevance
Mitochondrial Oxidative Damage
Application Stage
Basic Research
Applications
Oxidative stress marker in mitochondria.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565 nm
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Oxidative Stress; Mitochondrial Biology
Datasheet