Short Description
Measures citrate levels to evaluate TCA cycle flux.
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Citrate Assay Kit measures central carbon metabolism flux, validating drugs that target mitochondrial respiration and metabolic reprogramming in tumors. Direct measurement of citrate levels without separation steps simplifies the assessment of mitochondrial function in tissue lysates. This assay is critical for metabolic disease research, helping to identify therapeutic agents that modulate the TCA cycle.
Features
Direct measurement of citrate levels without separation steps; Key for assessing mitochondrial function and TCA cycle fluxes in metabolic disease research.
Process Relevance
TCA Cycle Flux Analysis
Application Stage
Discovery
Applications
Analyzes metabolic activity in mitochondria and tissue lysates.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm, or Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Serum, plasma, agriculture samples
Research Areas
Metabolic Disease; Oncology
Datasheet