Short Description
Measures fumarase enzyme activity.
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Fumarase Assay Kit determines enzymatic efficiency in the TCA cycle, used to study metabolic defects and screen for oncometabolite-related enzyme deficiencies. By validating mitochondrial integrity, it helps researchers understand the impact of drugs on the Krebs cycle in tissue lysates. This assay is particularly relevant for investigating metabolic diseases and developing targeted therapies for mitochondrial dysfunction.
Features
Focuses on enzymatic hydration activity; Validates mitochondrial integrity and drug effects on the Krebs cycle in tissue lysates.
Process Relevance
Mitochondrial Function Analysis
Application Stage
Basic Research
Applications
Used in analyzing mitochondrial function and TCA cycle enzyme defects.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565 nm
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Oncology; Metabolic Disease
Datasheet