OXPHOSight™ Glycolysis Assay Kit (CAT#: ITS-1125-WB3)

Quantifies glycolysis (L-lactate production) using a colorimetric (565 nm) or fluorimetric method.

OXPHOSight

Research-Grade Metabolic Assay Development

The OXPHOSight™ Glycolysis Assay Kit offers a high-throughput solution for quantifying glycolytic flux by measuring extracellular L-lactate production. Using a sensitive colorimetric method at 565 nm, this kit enables researchers to monitor metabolic shifts and screen for drug candidates targeting energy metabolism, providing essential kinetic data for cancer research and metabolic disease studies.

High Sensitivity and Precision: Employs an optimized enzyme-coupled chromogenic reaction to detect nanomolar lactate concentrations with exceptional reproducibility and specificity.
Streamlined Homogeneous Format: Features a "mix-and-read" procedure compatible with high-throughput screening, eliminating complex sample preparation or hazardous radioactive isotopes.
Versatile Sample Compatibility: Validated for diverse biological matrices, including cell culture supernatants, serum, and tissue lysates, ensuring broad experimental utility.

This kit enables real-time monitoring of metabolic shifts to facilitate lead compound screening and enzymatic pathway optimization.

Crucial for early-stage drug discovery, metabolic profiling in lead optimization, and rigorous in-process quality control.

Facilitating high-throughput screening for metabolic inhibitors, profiling cellular energy flux, assessing glucose metabolism in cancer models, and quantifying lactate across diverse biological supernatants.

Internal performance validation using reference standards under defined assay conditions.

Quantification of L-lactate as a surrogate marker for anaerobic glycolytic flux and cellular metabolism.

Absorbance at 565 nm

Chemical compounds

Oncology therapeutics, metabolic disorders, diabetes research, immunology and immunometabolism, cardiovascular physiology, etc.