OXPHOSight™ Glycolysis Assay Kit

CAT#: ITS-1125-WB3
Product Type: Assay Kit
Species: All Species
Target: Quantification of L-lactate as a surrogate marker for anaerobic glycolytic flux and cellular metabolism.
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Quantifies glycolysis (L-lactate production) using a colorimetric (565 nm) or fluorimetric method.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Glycolysis Assay Kit offers a high-throughput solution for quantifying glycolytic flux by measuring extracellular L-lactate production. Using a sensitive colorimetric method at 565 nm, this kit enables researchers to monitor metabolic shifts and screen for drug candidates targeting energy metabolism, providing essential kinetic data for cancer research and metabolic disease studies.
Features
High Sensitivity and Precision: Employs an optimized enzyme-coupled chromogenic reaction to detect nanomolar lactate concentrations with exceptional reproducibility and specificity.
Streamlined Homogeneous Format: Features a "mix-and-read" procedure compatible with high-throughput screening, eliminating complex sample preparation or hazardous radioactive isotopes.
Versatile Sample Compatibility: Validated for diverse biological matrices, including cell culture supernatants, serum, and tissue lysates, ensuring broad experimental utility.
Process Relevance
This kit enables real-time monitoring of metabolic shifts to facilitate lead compound screening and enzymatic pathway optimization.
Application Stage
Crucial for early-stage drug discovery, metabolic profiling in lead optimization, and rigorous in-process quality control.
Applications
Facilitating high-throughput screening for metabolic inhibitors, profiling cellular energy flux, assessing glucose metabolism in cancer models, and quantifying lactate across diverse biological supernatants.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Quantification of L-lactate as a surrogate marker for anaerobic glycolytic flux and cellular metabolism.
Detection Method
Absorbance at 565 nm
Sample Type
Chemical compounds
Research Areas
Oncology therapeutics, metabolic disorders, diabetes research, immunology and immunometabolism, cardiovascular physiology, etc.
Size
100 tests
Data Output Type
Quantitative measurement of L-lactate concentration (colorimetric, 565 nm) via endpoint or kinetic analysis.
Assay Time
30 min
Species
All Species
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Glycolysis is the fundamental metabolic pathway converting glucose to pyruvate. In pathological states like cancer, upregulated glycolysis fuels rapid proliferation, making lactate monitoring vital for therapeutic assessment.
Pathways
This assay monitors the terminal step of the Embden-Meyerhof-Parnas pathway. It reflects upstream glucose uptake and LDH activity, signaling critical shifts in the cellular bioenergetic state.
For Research Use Only | Not For Clinical Use
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