OXPHOSight™ Hexokinase Assay Kit

CAT#: ITS-1125-HM31
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Measures hexokinase activity.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Hexokinase Assay Kit evaluates the rate-limiting first step of glucose metabolism, supporting the discovery of metabolic modulators for diabetes and cancer treatment. By measuring glucose phosphorylation rates, researchers can validate the efficacy of compounds that alter glycolytic flux. This assay is crucial for metabolic reprogramming studies, helping to identify novel therapeutic targets in diseases characterized by altered glucose uptake.
Features
Measures rate-limiting glycolytic activity; Validates metabolic reprogramming agents in oncology and diabetes drug discovery.
Process Relevance
Cancer Metabolism Targeting
Application Stage
Target Validation
Applications
Glycolysis regulation studies.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565 nm
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Oncology; Diabetes & Obesity
Size
100 tests
Data Output Type
Kinetic (Enzyme Activity)
Assay Time
Results in 15 min
LOD
0.22 U/L
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Hexokinase catalyzes the phosphorylation of glucose to glucose-6-phosphate the first committed step of glycolysis. It regulates glucose entry into metabolic pathways and is often upregulated in cancer cells to support high glycolytic rates.
For Research Use Only | Not For Clinical Use
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