OXPHOSight™ Histamine Assay Kit

CAT#: ITS-1125-YL18
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Detects histamine levels using a colorimetric method.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ Histamine Assay Kit utilizes a robust colorimetric method for the quantification of histamine in biological fluids and experimental matrices. The system employs a specific enzymatic reaction that results in a measurable spectral change, providing a reliable alternative to complex chromatographic techniques. This specialized tool enables researchers to investigate immune signaling, allergic responses, and neurotransmission mechanisms, supporting comprehensive studies in cellular physiology and metabolic regulation.
Features
Features a direct enzymatic detection method for sensitive histamine quantification, enabling efficient research into immune response mechanisms and microbial fermentation without complex sample preparation.
Process Relevance
Monitoring histamine production to evaluate cellular signaling and biogenic amine metabolism in research.
Application Stage
Immunology research and inflammatory pathway analysis.
Applications
Determine histamine concentrations in biological fluids and cell supernatants to study immune signaling and metabolic degradation pathways.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565nm
Sample Type
Food, beverages, biological samples.
Research Areas
Inflammation Research; Immunology; Cell Signaling
Size
100 tests
Data Output Type
Quantitative concentration measurement
Assay Time
30 min
LOD
LOD: 2.5 µM
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
This biogenic amine is a critical mediator of inflammatory responses, gastric acid secretion, and neurotransmission. Its levels are frequently monitored to understand allergic reactions and physiological signaling in various organ systems.
Pathways
Histamine is synthesized from L-histidine by histidine decarboxylase and signals through four G-protein coupled receptors (H1-H4). It is a key mediator in the upstream initiation of the inflammatory response and allergic signaling cascades.
For Research Use Only | Not For Clinical Use
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