Short Description
Measures MDH activity via NADH oxidation.
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Malate Dehydrogenase Assay Kit offers kinetic UV determination for assessing gluconeogenic capacity and NADH shuttling in metabolic disease. This assay validates the activity of MDH, a key enzyme connecting the TCA cycle and glycolysis. It supports research into metabolic flexibility and the validation of drugs that target redox balance and energy production.
Features
Kinetic UV determination; Assessing gluconeogenic capacity and NADH shuttling in metabolic disease.
Process Relevance
Bioenergetics Research
Application Stage
Basic Research
Applications
Gluconeogenesis and malate-aspartate shuttle research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565 nm
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Metabolic Disease
Datasheet