OXPHOSight™ Malate Dehydrogenase Assay Kit

CAT#: ITS-1125-HM113
Product Type: Assay Kit
Species: All Species
Short Description
Measures MDH activity via NADH oxidation.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Malate Dehydrogenase Assay Kit offers kinetic UV determination for assessing gluconeogenic capacity and NADH shuttling in metabolic disease. This assay validates the activity of MDH, a key enzyme connecting the TCA cycle and glycolysis. It supports research into metabolic flexibility and the validation of drugs that target redox balance and energy production.
Features
Kinetic UV determination; Assessing gluconeogenic capacity and NADH shuttling in metabolic disease.
Process Relevance
Bioenergetics Research
Application Stage
Basic Research
Applications
Gluconeogenesis and malate-aspartate shuttle research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565 nm
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Metabolic Disease
Size
100 tests
Data Output Type
Kinetic (Enzyme Activity)
Assay Time
Results in 30 min
LOD
0.5 U/L
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Malate Dehydrogenase (MDH) converts malate to oxaloacetate. It is crucial for the malate-aspartate shuttle and gluconeogenesis reflecting the cell's ability to transport reducing equivalents.
For Research Use Only | Not For Clinical Use
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