Short Description
Measures oxaloacetate levels via a coupled enzymatic reaction.
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Oxaloacetate Assay Kit provides a sensitive metric for monitoring this critical TCA cycle intermediate, validating mitochondrial function and gluconeogenic flux. This assay is crucial for metabolic disease research, allowing for the assessment of drugs that modulate cellular bioenergetics and anaplerosis. By tracking oxaloacetate availability, researchers can better understand the metabolic shifts associated with diabetes and cancer.
Features
Simple room-temperature incubation; Highly specific for oxaloacetate with minimal interference from other keto acids.
Process Relevance
Metabolic Flux Analysis
Application Stage
Discovery / Basic Research
Applications
Monitoring TCA cycle dynamics in mitochondria and tissue lysates.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm, or Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Plasma, serum, tissue and culture media
Research Areas
Metabolic Disease; Bioenergetics
Datasheet