OXPHOSight™ Oxaloacetate Assay Kit

CAT#: ITS-1125-HM41
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Measures oxaloacetate levels via a coupled enzymatic reaction.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Oxaloacetate Assay Kit provides a sensitive metric for monitoring this critical TCA cycle intermediate, validating mitochondrial function and gluconeogenic flux. This assay is crucial for metabolic disease research, allowing for the assessment of drugs that modulate cellular bioenergetics and anaplerosis. By tracking oxaloacetate availability, researchers can better understand the metabolic shifts associated with diabetes and cancer.
Features
Simple room-temperature incubation; Highly specific for oxaloacetate with minimal interference from other keto acids.
Process Relevance
Metabolic Flux Analysis
Application Stage
Discovery / Basic Research
Applications
Monitoring TCA cycle dynamics in mitochondria and tissue lysates.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm, or Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Plasma, serum, tissue and culture media
Research Areas
Metabolic Disease; Bioenergetics
Size
100 tests
Data Output Type
Quantitative (Concentration)
Assay Time
Results in 20 min
LOD
OD 4 µM; FL 1 µM
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Oxaloacetate is a TCA cycle intermediate that reacts with acetyl-CoA to form citrate. It is also a substrate for gluconeogenesis and amino acid synthesis linking oxidative metabolism with biosynthetic pathways.
For Research Use Only | Not For Clinical Use
Online Inquiry