Short Description
Colorimetric or fluorometric detection of pyruvate concentrations.
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Pyruvate Assay Kit delivers dual colorimetric and fluorometric detection to quantify pyruvate, a central node in glycolysis and mitochondrial metabolism. This assay is indispensable for investigating the Warburg effect in oncology, helping to identify metabolic vulnerabilities in tumor cells. It supports metabolic flux analysis in drug discovery, enabling the assessment of compounds that target glycolytic regulation.
Features
Dual detection capabilities (Colorimetric/Fluorometric); Critical for metabolic flux analysis in Warburg effect studies.
Process Relevance
Cancer Metabolism Profiling
Application Stage
Lead Optimization
Applications
Evaluating glycolysis rates and metabolic flux in cancer cells.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm, or Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Oncology (Warburg Effect); Metabolic Disease
Datasheet