OXPHOSight™ Pyruvate Assay Kit

CAT#: ITS-1125-HM44
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Colorimetric or fluorometric detection of pyruvate concentrations.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Pyruvate Assay Kit delivers dual colorimetric and fluorometric detection to quantify pyruvate, a central node in glycolysis and mitochondrial metabolism. This assay is indispensable for investigating the Warburg effect in oncology, helping to identify metabolic vulnerabilities in tumor cells. It supports metabolic flux analysis in drug discovery, enabling the assessment of compounds that target glycolytic regulation.
Features
Dual detection capabilities (Colorimetric/Fluorometric); Critical for metabolic flux analysis in Warburg effect studies.
Process Relevance
Cancer Metabolism Profiling
Application Stage
Lead Optimization
Applications
Evaluating glycolysis rates and metabolic flux in cancer cells.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm, or Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Oncology (Warburg Effect); Metabolic Disease
Size
100 tests
Data Output Type
Quantitative (Concentration)
Assay Time
Results in 20 min
LOD
OD 2 µM; FL 0.2 µM
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Pyruvate is the end product of glycolysis. Its fate-conversion to lactate acetyl-CoA or oxaloacetate-determines whether the cell utilizes anaerobic fermentation or oxidative phosphorylation for energy.
For Research Use Only | Not For Clinical Use
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