OXPHOSight™ Pyruvate Dehydrogenase Assay Kit

CAT#: ITS-1125-HM122
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Measures PDH complex activity.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Pyruvate Dehydrogenase Assay Kit is compatible with intact mitochondria or lysates, validating modulators of PDH kinases and phosphatases in metabolic disease. This assay measures the activity of the PDH complex, the gatekeeper of glucose oxidation. It supports the development of drugs that restore metabolic flexibility in diabetes and heart failure.
Features
Intact mitochondria or lysate compatible; Validates PDH kinases/phosphatases modulators in metabolic disease.
Process Relevance
Metabolic Switch Research
Application Stage
Target Validation
Applications
Metabolic flexibility and mitochondrial disease study.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565 nm
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Metabolic Disease; Diabetes
Size
100 tests
Data Output Type
Kinetic (Enzyme Activity)
Assay Time
Results in 20 min
LOD
0.87 U/L
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
The Pyruvate Dehydrogenase Complex (PDH) links glycolysis to the TCA cycle. Its regulation is a key determinant of fuel selection (glucose vs. fat oxidation) in the heart and skeletal muscle.
For Research Use Only | Not For Clinical Use
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