Short Description
Measures PDH complex activity.
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Pyruvate Dehydrogenase Assay Kit is compatible with intact mitochondria or lysates, validating modulators of PDH kinases and phosphatases in metabolic disease. This assay measures the activity of the PDH complex, the gatekeeper of glucose oxidation. It supports the development of drugs that restore metabolic flexibility in diabetes and heart failure.
Features
Intact mitochondria or lysate compatible; Validates PDH kinases/phosphatases modulators in metabolic disease.
Process Relevance
Metabolic Switch Research
Application Stage
Target Validation
Applications
Metabolic flexibility and mitochondrial disease study.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565 nm
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Metabolic Disease; Diabetes
Datasheet