OXPHOSight™ Sialic Acid Assay Kit

CAT#: ITS-1125-YL27
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Measures sialic acid using a colorimetric method.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ Sialic Acid Assay Kit provides a sensitive colorimetric method for quantifying total sialic acids in biological fluids and cell lysates. The system utilizes an enzymatic process that releases and oxidizes sialic acids to form a colored product. This specialized tool enables researchers to investigate cell surface glycosylation and glycan metabolism, supporting fundamental studies in cell signaling, molecular recognition, and structural biochemistry.
Features
Employs a specific enzymatic oxidation for the rapid colorimetric quantification of sialic acids, facilitating research in glycobiology and the analysis of cell surface modifications.
Process Relevance
Monitoring sialic acid levels to evaluate glycosylation patterns and cell surface characteristics in vitro.
Application Stage
Glycobiology research and cell surface characterization.
Applications
Measure sialic acid content in glycoproteins and biological fluids to investigate glycosylation patterns and cellular recognition mechanisms.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 549nm, or Fluorescence Detection (Ex 555 nm / Em 585 nm)
Sample Type
Serum, plasma, biological fluids.
Research Areas
Glycobiology; Cell Signaling; Immunology
Size
100 tests
Data Output Type
Quantitative concentration measurement
Assay Time
2.5 hrs
LOD
LOD: OD, FL: 5, 0.5 µM
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
24 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
These acidic monosaccharides are typically found at the terminal positions of glycoproteins and glycolipids. They play critical roles in cell-cell recognition, molecular trafficking, and the modulation of the immune system.
Pathways
Formate is a central intermediate in the folate-mediated one-carbon metabolism pathway, connecting mitochondrial respiration to cytoplasmic biosynthetic reactions. It serves as an upstream precursor for purine and thymidylate synthesis.
For Research Use Only | Not For Clinical Use
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