Short Description
Measures sialic acid using a colorimetric method.
Development Stage
Research-Grade Metabolic Assay Development
Description
This OXPHOSight™ Sialic Acid Assay Kit provides a sensitive colorimetric method for quantifying total sialic acids in biological fluids and cell lysates. The system utilizes an enzymatic process that releases and oxidizes sialic acids to form a colored product. This specialized tool enables researchers to investigate cell surface glycosylation and glycan metabolism, supporting fundamental studies in cell signaling, molecular recognition, and structural biochemistry.
Features
Employs a specific enzymatic oxidation for the rapid colorimetric quantification of sialic acids, facilitating research in glycobiology and the analysis of cell surface modifications.
Process Relevance
Monitoring sialic acid levels to evaluate glycosylation patterns and cell surface characteristics in vitro.
Application Stage
Glycobiology research and cell surface characterization.
Applications
Measure sialic acid content in glycoproteins and biological fluids to investigate glycosylation patterns and cellular recognition mechanisms.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 549nm, or Fluorescence Detection (Ex 555 nm / Em 585 nm)
Sample Type
Serum, plasma, biological fluids.
Research Areas
Glycobiology; Cell Signaling; Immunology
Datasheet