Short Description
Quantifies urokinase activity and screens inhibitors using a fluorometric method.
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Urokinase Inhibitor Screening Assay Kit provides a rapid and sensitive fluorimetric platform for identifying potent inhibitors of urokinase plasminogen activator. This high-throughput compatible system is crucial for drug discovery targeting extracellular matrix degradation and tumor progression, offering reliable kinetic data to accelerate lead optimization. By utilizing a specific fluorimetric substrate to monitor enzyme activity, it enables precise screening and safety profiling of thrombolytic and anti-metastatic drug candidates in pharmaceutical research.
Features
High-Resolution Sensitivity: Employs a specific fluorogenic substrate to detect subtle inhibitory potencies with a wide dynamic range and minimal background interference.
Robust HTS Compatibility: Optimized for seamless integration into 96-well or 384-well microplate formats, ensuring high-throughput screening consistency and rapid data acquisition.
Streamlined Homogeneous Workflow: Features a simple "mix-and-read" protocol that eliminates complex wash steps, significantly reducing experimental variability and hands-on laboratory time.
Validated Specificity: Engineered to minimize cross-reactivity with related proteases, providing confidence that observed inhibitory effects are directly attributable to urokinase modulation.
Process Relevance
This kit accelerates drug discovery by providing a standardized platform to validate uPA inhibitory potency and kinetic profiles.
Application Stage
Ideal for lead compound identification, structure-activity relationship (SAR) studies, and preclinical pharmaceutical quality assessment.
Applications
Enabling high-throughput screening of chemical libraries and natural extracts to identify potent uPA inhibitors for oncology and wound healing research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Specifically targets human urokinase (uPA) proteolytic activity within purified or complex biological systems.
Detection Method
Fluorescence Detection (Ex 380 nm / Em 450 nm)
Sample Type
Chemical compounds
Research Areas
Oncology therapeutics, cardiovascular biology, fibrinolysis, wound healing, extracellular matrix remodeling, etc.
Datasheet