Short Description
Measures the activity of Xanthine Oxidase enzyme.
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Xanthine Oxidase Assay Kit measures the activity of this superoxide-generating enzyme, essential for screening potential gout therapeutics and antioxidants. By validating XO inhibitors, this assay helps reduce oxidative stress and uric acid production in metabolic disease models. It is a vital tool for mechanistic toxicology studies investigating ischemia-reperfusion injury and vascular inflammation.
Features
Kinetic assay format; Essential for screening potential gout therapeutics and oxidative stress modulators.
Process Relevance
Enzyme Inhibitor Screening
Application Stage
Hit-to-Lead Screening
Applications
Evaluating oxidative stress mechanisms and XO inhibitor efficacy.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm, Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Cell lysate, serum, and other biological samples
Research Areas
Rheumatology; Oxidative Stress
Datasheet