OXPHOSight™ Xanthine Oxidase Assay Kit

CAT#: ITS-1125-HM51
Product Type: Assay Kit
Species: All Species
Brand: OXPHOSight
Development Stage: Research-Grade Metabolic Assay Development
Short Description
Measures the activity of Xanthine Oxidase enzyme.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Xanthine Oxidase Assay Kit measures the activity of this superoxide-generating enzyme, essential for screening potential gout therapeutics and antioxidants. By validating XO inhibitors, this assay helps reduce oxidative stress and uric acid production in metabolic disease models. It is a vital tool for mechanistic toxicology studies investigating ischemia-reperfusion injury and vascular inflammation.
Features
Kinetic assay format; Essential for screening potential gout therapeutics and oxidative stress modulators.
Process Relevance
Enzyme Inhibitor Screening
Application Stage
Hit-to-Lead Screening
Applications
Evaluating oxidative stress mechanisms and XO inhibitor efficacy.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm, Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Cell lysate, serum, and other biological samples
Research Areas
Rheumatology; Oxidative Stress
Size
100 tests
Data Output Type
Kinetic (Enzyme Activity)
Assay Time
Results in 20 min
LOD
OD 0.03 U/L; FL 0.01 U/L
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Xanthine Oxidase catalyzes the oxidation of hypoxanthine to xanthine and uric acid generating superoxide radicals. It is a key source of oxidative stress in reperfusion injury and a therapeutic target for gout.
For Research Use Only | Not For Clinical Use
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