PC-9 In Vitro (3)H-thymidine Incorporation Assay (Cell Proliferation)

This assay is to provide PC-9-based In Vitro (3)H-thymidine Incorporation Assay (Cell Proliferation) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

PC-9

Human

PC-9 is lung adenocarcinoma cell line with a deletion in exon 19 of the EGFR gene that exhibits high sensitivity to TKIs. The PC-9G cell line (PC-9 cells resistant to gefitinib) was established by long-term exposure to gefitinib, and was shown to harbor the EGFR T790M mutation.

Lung Cancer

Oncology

In Vitro (3)H-thymidine Incorporation Assay (Cell Proliferation)

The 3H-thymidine incorporation assay is one such assay that can detect newly synthesized DNA in cells. Cultured cells are mixed with 3H-thymidine (radioactive) and kept for a desired incubation period. Newly replicated DNA strands can incorporate 3H-thymidine during mitosis. A scintillation counter is necessary to detect the output. Direct measurement of proliferating cells is the main advantage of this assay. However, use of harmful radioactive substances and need of special equipment are considered as main disadvantages.

Cell Viability/Cytotoxicity and Antiproliferative Assays

Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.

Cell Proliferation Assay

Cell-based Assays Based on DNA Synthesis