PC-9 In Vitro Mitotic Index Assay (Cell Proliferation)

This assay is to provide PC-9-based In Vitro Mitotic Index Assay (Cell Proliferation) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

PC-9

Human

PC-9 is lung adenocarcinoma cell line with a deletion in exon 19 of the EGFR gene that exhibits high sensitivity to TKIs. The PC-9G cell line (PC-9 cells resistant to gefitinib) was established by long-term exposure to gefitinib, and was shown to harbor the EGFR T790M mutation.

Lung Cancer

Oncology

In Vitro Mitotic Index Assay (Cell Proliferation)

Mitotic index assay is one of the old assays used to determine the number of cells that are undergoing mitosis in a specified cell population using monoclonal antibodies specific for phosphorylated histone H3 (ser28). Phosphorylated histone H3 (ser28) is considered as a main marker for mitosis and this specific phosphorylation can be utilized to calculate the mitotic index in a specified cell population.

Cell Viability/Cytotoxicity and Antiproliferative Assays

Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.

Cell Proliferation Assay

Cell-based Assays Based on DNA Synthesis