PC-9 In Vitro Reactive Nitrogen Species Assay (Oxidative Stress)

This assay is to provide PC-9-based In Vitro Reactive Nitrogen Species Assay (Oxidative Stress) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

PC-9

Human

PC-9 is lung adenocarcinoma cell line with a deletion in exon 19 of the EGFR gene that exhibits high sensitivity to TKIs. The PC-9G cell line (PC-9 cells resistant to gefitinib) was established by long-term exposure to gefitinib, and was shown to harbor the EGFR T790M mutation.

Lung Cancer

Oncology

In Vitro Reactive Nitrogen Species Assay (Oxidative Stress)

The production of reactive nitrogen species can also occur as a result of oxidative stress. Levels of nitrite (NO2-) and nitrate (NO3-) can be measured in cell lysates, plasma and urine as an oxidative stress marker and several nitrate/nitrite assay kits have been developed to measure nitrite (NO2-) and nitrate (NO3-) levels.

Oxidative Stress Assays

Disturbance between the production of reactive oxygen species (ROS), free radicals and antioxidant mechanisms is defined as the oxidative stress, or more precisely, it is an imbalance between the oxidant and antioxidant state in cells. This imbalance can cause harmful effects to cells and biomolecules, which ultimately causes adverse effects in the whole organism. Oxidative imbalance can target important proteinsand lipids in cells, which can increase the risk of developing a cancer. On the other hand, increased ROS production in cancer cells by certain cancer drugs can also arrest cancer cell cycle and cause senescence and apoptosis through oxidative stress.

Markers Thiobarbituric Acid Reactive Substances