PC3 In Vitro Cell Membrane Integrity-based Apoptosis Assay (FCM)

This assay is to provide PC3-based In Vitro Cell Membrane Integrity-based Apoptosis Assay (FCM) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

PC3

Human

PC3 (PC-3) is a human prostate cancer cell line used in prostate cancer research and drug development. PC3 cells are useful in investigating biochemical changes in advanced prostate cancer cells and in assessing their response to chemotherapeutic agents. PC3 cells are also used to study viral infection in mammalian cells that exhibit an immune response.

PC-3

Prostate Cancer

Oncology

In Vitro Cell Membrane Integrity-based Apoptosis Assay (FCM)

PC3-cell based In Vitro Cell Membrane Integrity-based Apoptosis Assay (FCM)

FCM is one of the best and accurate automated tecqniques used for the quantification of apoptosis. FCM has been widely used in cancer drug discovery approaches to detect apoptosis. FCM can be used to monitor integrity of cancer cell membranes. Dyes such as PI, fluorescein diacetate, carboxyfluorescein diacetate and TO-PRO-3 are commonly used examples for this purpose in FCM.

Detection of Apoptosis Assays

Apoptosis (programmed cell death) plays a vital role in embryonic development, homeostasis, functioning of immune system and wound repair. The ability to evade induction of apoptosis has been used by cancer cells to survive against host defense mechanisms. The molecular mechanisms involved in cancer cell apoptosis have been well documented and it involves certain biochemical events such as DNA fragmentation, chromatin condensation, cell organelle degradation and protein cleavage, etc. The extrinsic and intrinsic (mitochondrial) pathways are the two major pathways involved in apoptosis. With the available techniques and assays, a number of apoptosis inducing agents (natural compounds, synthetic compounds, nano-formulations, peptides and enzymes) in many cancer cells have been identified. Selection of an assay for apoptosis detection is based on factors such as apoptotic pathway, nature of drug, cell type being used and the method of analysis.

Flow Cytometry to Detect Apoptosis Assays

Flow Cytometry Assay