Rabbit CXCL9 ELISA Kit, Lot 21AO-780 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Rabbit CXCL9 in Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate.

Rabbit MIG ELISA Kit.

ELISA

Optimal working dilution should be determined by the investigator.

CXCL9

Rabbit

Colorimetric

Sandwich ELISA

Quantitative

Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Distilled or deionized water
Precision pipettes to deliver 2 μL to 1 μL volumes
Adjustable 1-25 μL pipettes for reagent preparation
100 μL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Pre-coated

6 months

For Research Use only

Monokine Induced By Interferon Gamma (MIg)

CMK; Humig; MIG; SCYB9; crg-10; BB139920; Mig; MuMIG; Scyb9; C-X-C motif chemokine ligand 9; chemokine (C-X-C motif) ligand 9; CXCL9; Cxcl9

U3KNX2

1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 μL of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 μL of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 μL of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 μL of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 μL of Stop Solution to each well.
11.Read at 450 nm immediately.