Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide Raji-based In Vitro WST Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
Raji
Target Cell Organism
Human
Target Cell Background
Raji cells were derived from the B-lymphocytes of an 11-year-old Nigerian Burkitt's lymphoma male patient in 1963 by R.J.V. Pulvertaft and was further worked on by B.O. Osunkoya (University College Hospital, Ibadan, Nigeria). The Raji cell line is categorized as lymphoblast-like.
Related Diseases
B Lymphoma
Research Area
Oncology
Assay Name
In Vitro WST Assay (Cell Viability)
Short Description
Raji-cell based In Vitro WST Assay (Cell Viability)
Assay Description
Apart from the MTT tetrazolium, other tetrazolium compounds such as WST (reagent series) can also generate formazan upon reduction by oxidoreductase or dehydrogenase. Unlike the normal MTT assay, assays with these regents (MTS, XTT and WST) do not require formazan solubilization step as solubilization occurs in the cell medium itself. Therefore, XTT, WST and MTS reagents can be used to assay viability of cells, which do not require a surface to attach.
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Viability Assay
Assay MOA
Cell-based Viability Assays Based on Cellular Enzymes and Proteins