Short Decsription
The kit is designed for in vitro quantitative measurement of Rat IL1A in Cell Culture Supernatant, Plasma, Serum, Tissue Lysate.
Description
For quantitative detection of IL-1α in Rat serum, body fluids, tissue lysates or cell culture supernates.
Comment
Anti-IL-1α polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-1α polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of IL-1α can be calculated.
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Plasma, Serum, Tissue Lysate
Datasheet