Rat PVRL2 ELISA Kit, Lot 21AO-227 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Rat PVRL2 in Plasma, Serum.

The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PVRL2 in Rat serum, plasma and other biological fluids

ELISA

ELISA Plate: The just opened ELISA Plate may appear water-like substance, which is normal and will not have any impact on the experiment results.
Add Sample: The interval of sample adding between the first well and the last well should not be too long, otherwise will cause different pre-incubation time, which will significantly affect the experiment's accuracy and repeatability.
Incubation: To prevent evaporation and ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary.
Washing: The wash procedure is critical.
Reagent Preparation: As the volume of Detection Ab and HRP Conjugate is very small, liquid may adhere to the tube wall or tube cap when being transported. You better hand-throw it or centrifugal it for 1 minute at 1000rpm. Please pipette the solution for 4-5 times before pippeting.
Substrate: Substrate Solution is easily contaminated. Please protect it from light.

Information on standard material: The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).
Information on reagents: Reagents include 1 M SO2. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.
Information on antibodies: The provided antibodies and their host vary in different kits. All antibodies are affinity purified.

PVRL2

Rat

Colorimetric

Sandwich ELISA

Quantitative

Plasma, Serum

This kit recognizes natural and recombinant Rat PVRL2. No significant cross-reactivity or interference between Rat PVRL2 and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between Rat PVRL2 and all the analogues.

No significant cross-reactivity or interference between rat Poliovirus Receptor Related Protein 2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Human bFGF and all the analogues, therefore, cross reaction may still exist.

Micro ELISA Plate
Reference Standard
Concentrated Biotinylated Detection Ab
Concentrated HRP Conjugate
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer (25×)
Substrate Reagent
Stop Solutio
Plate Sealer
Product Description
Certificate of Analysis

Microplate reader with 450nm wavelength filter
High-precision transferpettor, EP tubes and disposable pipette tips
37°C Incubator
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer

0.094 ng/mL

100 μL

Pre-coated

Bring all reagents and samples to room temperature for 20 minutes before use.
Wash Buffer
Standards
Preparation of Biotin-labeled Antibody Working Solution
Preparation of HRP-Streptavidin Conjugate (SABC) Working Solution

1.Bring all reagents and samples to room temperature (18 - 25°C) before use.
2.Add 100 µl of each standard and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4°C with gentle shaking.
3.Discard the solution and wash 4 times with 1x Wash Solution.
4.Add 100 µl of 1x prepared biotinylated antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
5.Discard the solution. Repeat the wash as in step 3.
6.Add 100 µl of prepared Streptavidin solution to each well. Incubate for 45 minutes at room temperature with gentle shaking.
7.Discard the solution. Repeat the wash as in step 3.
8.Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
9.Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.

All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination.

4 °C/-20 °C

The unopened kit can be stored at 4°C for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.

The unopened kit can be stored at 4°C for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.

For Research Use only

Poliovirus Receptor Related Protein 2

prr2; AI325026; AI987993; Cd112; MPH; Pvr; Pvs; nectin-2; CD112; HVEB; PRR2; PVRR2; nectin cell adhesion molecule 2; Nectin2; NECTIN2

308417

Q5FVC5

Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization

The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PVRL2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PVRL2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm.