Rhesus Monkey IL12 ELISA Kit, Lot 21AO-711 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P622
Product Type: ELISA Kit
Target: IL12
Short Description
The kit is designed for in vitro quantitative measurement of Rhesus Monkey IL12 in Cell Culture Supernatant, Plasma, Serum.
Description
Rhesus Monkey IL-12 p70 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Applications
ELISA
Application Notes
Recommended Dilution for serum and plasma samples: 2-fold
Target
IL12
Reactivity
Rhesus Monkey
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Plasma, Serum
Specificity
This ELISA antibody pair detects Rhesus Monkey IL-12 p70 and human IL-12 p70. Other species not determined.
Components
Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate
Material not included
Distilled or deionized water
Precision pipettes to deliver 2 μL to 1 μL volumes
Adjustable 1-25 μL pipettes for reagent preparation
100 μL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis
Sensitivity
40 pg/mL
Sample Volume
100 μL
Plate
Pre-coated
Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Note
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Restrictions
For Research Use only
Alternative Name
Interleukin 12
Synonyms
CLMF; CLMF2; IL-12B; NKSF; NKSF2; p40; Il-12b; Il12p40; Il-12p40; Il12; IL-12p40; IL-12; LOC100217488; IL-12p35; Il-12a; Ll12a; p35; IL12p40; IL12B; IL-12 p35; interleukin 12B; interleukin 12A; interleukin 12b; interleukin 12a; Interleukin 12 level; IL12B; IL12A; Il12b; Il12a; IL12; il12a
Background
Gene Names: IL12A
Protein names: Interleukin-12 subunit alpha (IL-12A) (Cytotoxic lymphocyte maturation factor 35 kDa subunit) (CLMF p35) (IL-12 subunit p35)
Gene ID
703205
UniProt
P48091
Pathways
JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
Protocol
1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 μL of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 μL of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 μL of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 μL of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 μL of Stop Solution to each well.
11.Read at 450 nm immediately.
For Research Use Only | Not For Clinical Use
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