Short Description
Measures free choline levels.
Development Stage
Research-Grade Signal Pathway Assay Development
Description
The SigPath™ Choline Assay Kit assesses the availability of precursors for membrane phospholipids and neurotransmitters, serving as a key tool for neuroprotective drug discovery. By validating the effects of compounds on phospholipid synthesis and cholinergic signaling, it aids in the development of cognitive enhancers and membrane-stabilizing agents. This enzyme-coupled reaction provides the high sensitivity required for analyzing serum and tissue samples in neuronal models.
Features
Enzyme-coupled reaction provides high sensitivity; Validates phospholipid synthesis inhibitors and cholinergic drugs in neuronal models.
Process Relevance
Neuroprotective Drug Discovery
Application Stage
Hit-to-Lead Screening
Applications
Evaluates phospholipid synthesis and cholinergic pathway precursors.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm, or Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Serum, plasma, urine, saliva, milk, tissue, cells samples
Research Areas
Neuroscience; Cell Biology
Datasheet