SigPath™ Choline Assay Kit

CAT#: ITS-1125-HM14
Product Type: Assay Kit
Species: All Species
Brand: SigPath
Development Stage: Research-Grade Signal Pathway Assay Development
Short Description
Measures free choline levels.
Brand
SigPath
Development Stage
Research-Grade Signal Pathway Assay Development
Description
The SigPath™ Choline Assay Kit assesses the availability of precursors for membrane phospholipids and neurotransmitters, serving as a key tool for neuroprotective drug discovery. By validating the effects of compounds on phospholipid synthesis and cholinergic signaling, it aids in the development of cognitive enhancers and membrane-stabilizing agents. This enzyme-coupled reaction provides the high sensitivity required for analyzing serum and tissue samples in neuronal models.
Features
Enzyme-coupled reaction provides high sensitivity; Validates phospholipid synthesis inhibitors and cholinergic drugs in neuronal models.
Process Relevance
Neuroprotective Drug Discovery
Application Stage
Hit-to-Lead Screening
Applications
Evaluates phospholipid synthesis and cholinergic pathway precursors.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm, or Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Serum, plasma, urine, saliva, milk, tissue, cells samples
Research Areas
Neuroscience; Cell Biology
Size
100 tests
Data Output Type
Quantitative (Concentration)
Assay Time
Results in 30 min
LOD
OD 1 µM; FL 0.2 µM
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Choline is an essential nutrient required for the synthesis of acetylcholine and phosphatidylcholine. It plays a key role in lipid transport and methyl group metabolism influencing liver function and brain development.
For Research Use Only | Not For Clinical Use
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