SigPath™ Human/Mouse LKB1(S431) Phosphorylation ELISA Kit

CAT#: ITS-1125-WB10
Product Type: Assay Kit
Species: Human, mouse, rat
Target: Human and mouse liver kinase B1 (LKB1) phosphorylated specifically at the serine 431 residue.
Brand: SigPath
Development Stage: Research-Grade Signal Pathway Assay Development
Short Description
Quantifies LKB1 (Ser431) phosphorylation levels using a sandwich ELISA method.
Brand
SigPath
Development Stage
Research-Grade Signal Pathway Assay Development
Description
The SigPath™ Human/Mouse LKB1(S431) Phosphorylation ELISA Kit provides a rapid and sensitive solution for measuring pLKB1(S431) levels directly in cultured cells. By normalizing phosphorylation to total protein content within the same well, this high-throughput system eliminates cell lysis steps, ensuring precise data for screening inhibitors and studying tumor suppressor signaling pathways in drug discovery.
Features
Superior Sensitivity: Employs high-affinity antibody pairs for precise detection of endogenous phospho-LKB1 levels in diverse cell lysates and tissue samples.
Dual-Species Reactivity: Offers validated performance across both human and mouse models, ensuring seamless cross-species experimental consistency and reliable data translation.
Optimized Assay Format: Streamlined sandwich ELISA protocol minimizes hands-on time while delivering robust, reproducible results through standardized, pre-coated microplate components.
Phospho-Specific Accuracy: Specifically targets the Serine 431 residue, eliminating cross-reactivity with non-phosphorylated isoforms for high-fidelity signaling pathway analysis.
Process Relevance
Quantifying LKB1 phosphorylation status to evaluate AMPK-related metabolic regulation and ensure consistency in therapeutic signaling modulation assays.
Application Stage
Translational research, high-throughput drug screening, and validation of metabolic signaling pathway inhibitors during preclinical development stages.
Applications
Quantifing S431 phosphorylation in human/mouse cell lysates for kinase assays, metabolic profiling, and evaluating therapeutic efficacy in oncology and diabetic research models.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Human and mouse liver kinase B1 (LKB1) phosphorylated specifically at the serine 431 residue.
Detection Method
Fluorescence Detection (Ex 530 nm / Em 585 nm or Ex 360 nm / Em 456 nm)
Sample Type
Cells
Research Areas
Oncology therapeutics, metabolic disorders, cell cycle regulation, autophagy, AMPK signaling pathways, etc.
Size
100 tests
Data Output Type
Quantitative measurement of relative protein phosphorylation levels via endpoint colorimetric absorbance.
Assay Time
6.5 h
Species
Human, mouse, rat
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
LKB1 acts as a master upstream kinase regulating cellular energy homeostasis. Phosphorylation at S431 by PKA or p90RSK modulates its tumor-suppressive functions and downstream AMPK activation.
Pathways
LKB1 participates in the AMPK/mTOR signaling axis, integrating signals from the cAMP/PKA pathway. It bridges extracellular stimuli to intracellular metabolic shifts, influencing growth and survival.
For Research Use Only | Not For Clinical Use
Online Inquiry