Short Description
Quantifies rpS6 (Ser235/236) phosphorylation levels in whole cells using a colorimetric ELISA method.
Development Stage
Research-Grade Signal Pathway Assay Development
Description
The SigPath™ Human/Mouse RPS6(S235/236) Phosphorylation ELISA Kit offers a streamlined, cell-based solution for quantifying ribosomal protein S6 activation within intact cells. By eliminating the need for cell lysis and incorporating total protein normalization, this high-throughput compatible system ensures high precision and reproducibility. It is an essential tool for screening kinase inhibitors and studying mTOR/S6K signaling pathways in pharmaceutical research and drug discovery.
Features
High-Throughput Compatibility: This optimized 96-well sandwich format enables rapid screening of multiple cell lysates, effectively replacing time-consuming Western blot protocols.
Exceptional Site Specificity: Utilizing premium phospho-specific antibodies ensures the precise detection of dual phosphorylation at Ser235/236 without cross-reactivity to non-phosphorylated targets.
Species-Versatile Performance: Validated for high-fidelity detection in both human and mouse samples, supporting seamless transition from clinical research to murine models.
Complete Quantitative Integrity: The kit includes pre-coated plates and specialized lysis buffers, delivering a robust dynamic range for exact protein quantification.
Process Relevance
This kit facilitates rapid quantification of pathway modulation to accelerate lead optimization and confirm therapeutic mechanism of action.
Application Stage
Essential for high-throughput drug screening, pharmacodynamic profiling, and verifying target engagement during preclinical lead development and optimization.
Applications
Ideal for cell-based signaling assays, kinase inhibitor screening, and monitoring translational control in oncological, metabolic, and neurobiological research across diverse tissue lysates.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Ribosomal protein S6 (RPS6) dually phosphorylated at the serine 235 and serine 236 residues.
Detection Method
Fluorescence Detection (Ex 530 nm / Em 585 nm or Ex 360 nm / Em 458 nm)
Research Areas
Oncology therapeutics, metabolic disorders, translational regulation, neurobiology, PI3K/Akt/mTOR pathway signaling, etc.
Datasheet