STAT3 Quantification Service
The STAT3 Quantification Service is a specialized bioassay that provides precise measurement and analysis of signal transducer and Activator of Transcription 3 (STAT3) protein levels, often utilized in research and clinical settings to investigate various diseases, including cancer and autoimmune disorders.
STAT3 Signaling Pathway
The STAT3 signaling pathway is a crucial cellular communication route that mediates various biological processes, including cell growth, survival, differentiation, and immune response. Activated by a range of cytokines and growth factors, such as interleukin-6 (IL-6) and epidermal growth factor (EGF), this pathway begins with the binding of ligands to their respective receptors, leading to the activation of Janus kinases (JAKs). These JAKs phosphorylate the cytoplasmic tail of the receptors and subsequently activate signal transducer and activator of transcription 3 (STAT3) through phosphorylation at a specific tyrosine residue. Once activated, STAT3 dimerizes, translocates to the nucleus, and binds to specific DNA response elements to modulate gene expression. Dysregulation of STAT3 signaling has been implicated in various diseases, including cancer and autoimmune disorders, highlighting its potential as a therapeutic target.
STAT3 Quantification Service at Creative Biolabs
Our STAT3 quantification service offers precise and reliable measurement of the signal transducer and STAT3 protein. Utilizing advanced techniques such as Enzyme-Linked Immunosorbent Assay (ELISA) and Western blotting, our service provides you with quantitative data on STAT3 expression levels and activation states, enabling them to investigate the protein's involvement in cancer, inflammation, and other diseases. By delivering accurate and timely data, the STAT3 quantification service empowers customers to advance their research and contribute valuable insights to the understanding of STAT3-related pathways.
Process of our service:
Methods of assessment:
-
ELISA
Specific antibodies are used to detect and measure STAT3 protein concentrations in lysates or serum.
-
Western Blotting
This technique can be used for the detection and quantification of STAT3 protein.
-
RT-qPCR (Real-Time Quantitative PCR)
While this method doesn't directly measure STAT3 protein, it quantifies STAT3 mRNA levels.
-
Flow Cytometry
Can be used to assess the activation state of STAT3 in cells by utilizing fluorescently labeled antibodies that bind to phosphorylated forms of STAT3.
-
Mass Spectrometry
An advanced technique to quantify proteins and gain insights into post-translational modifications.
-
Immunohistochemistry (IHC)
Used for localized detection of STAT3 in tissue samples, providing qualitative and semi-quantitative data on its expression within specific cell types
Applications of STAT3 Quantification
Quantification of STAT3 is significant for various applications in research and clinical settings. Here are some key applications:
Frequently Asked Question
Q1: What is the timeline for STAT3 activation/deactivation assessment service?
A1: The timeline for STAT3 activation/deactivation assessment services can vary based on the specific methods you need. Here's a general outline of what the timeline might look like:
|
Sample Collection
|
This can take anywhere from a few minutes to several hours, depending on the method of sample collection.
|
|
Sample Processing
|
After collection, samples often need to be processed (e.g., centrifugation, cell lysis) before analysis. This can take a few hours.
|
|
Assay Preparation
|
Depending on the technique used (such as Western blotting, ELISA, or flow cytometry), preparing the assays might take from a few hours to a day.
|
|
Analysis
|
The actual analysis can vary significantly.
|
|
Data Interpretation
|
Once the analysis is complete, interpreting the results can take additional time, usually a day or two.
|
|
Reporting
|
Generating the final report and providing results to the requesting can take an additional few days.
|
Overall, the time period that may be required throughout the inspection process depends on the factors mentioned above, and you can contact our technicians for a more detailed project arrangement.
Contact Us
At Creative Biolabs, we are dedicated to delivering high-quality research solutions, including our specialized STAT3 Quantification Service. Our team of experts employs advanced methodologies to ensure precise and reliable quantification of STAT3, supporting your research and therapeutic development needs. Whether you are looking to enhance your understanding of signaling pathways or investigate therapeutic targets, we invite you to contact us now.
For Research Use Only | Not For Clinical Use
Download our brochure
