Introduction

Signal Transducer and Activator of Transcription 5 (STAT5) proteins are pivotal in various cellular processes such as immune regulation, cell proliferation, and survival. The two paralogs, STAT5a and STAT5b, exhibit a high degree of similarity but perform distinct roles within the cell. Dysregulation of STAT5 activity is implicated in several hematopoietic malignancies, autoimmune disorders, and allergic inflammations. Creative Biolabs specializes in the robust assessment of STAT5 activation and deactivation, employing state-of-the-art technologies for comprehensive analysis.

Role of STAT5 in Immune-Oncology

STAT5 activation is initiated via cytokines and growth factors binding to their respective receptors, followed by the phosphorylation of STAT5 by Janus kinases (JAKs). This cascade results in the dimerization and nuclear translocation of STAT5, which influences the transcription of target genes. Abnormal activation or mutations in STAT5, particularly STAT5b, are linked to various hematopoietic malignancies such as acute lymphoblastic leukemia (ALL), chronic myelogenous leukemia (CML), and large granular lymphocyte (LGL) leukemia. In the immune system, STAT5 plays a crucial role in lymphocyte development and function, contributing to both physiological and pathological processes.

Fig.1 Schematic of STAT5 signaling with core cancer pathways.¹

Assessment of STAT5 Activation/Deactivation at Creative Biolabs

At Creative Biolabs, we provide an advanced assay for quantifying phosphorylated STAT5 versus total STAT5, enabling precise measurement of STAT5 activation and deactivation. This assay offers high sensitivity and robustness, suitable for multi-parallel and high-throughput applications, which is vital for elucidating the complex signaling networks in immune-oncology. By employing the STAT5 assessment service, we can evaluate specific phosphorylation events within cell populations, allowing researchers to monitor the activation status of STAT5 comprehensively.

Service Content

Our STAT5 activation/deactivation service encompasses the following:

• Quantitative Analysis:
  Measurement of STAT5 phosphorylation status in response to various stimuli (e.g., cytokines, growth factors).
  Evaluation of total STAT5 levels to ensure assay accuracy.
• High-Throughput Screening:
  Large-scale screening capabilities to identify inhibitors or activators of STAT5.
  Customizable assay formats tailored to specific research needs.
• Data Interpretation and Reporting:
  Detailed analysis reports including comprehensive data interpretation.
  Recommendations for downstream applications and potential therapeutic interventions.

Application of Assessment for STAT5 Activation/Deactivation

The assessment of STAT5 activity is critical in several research and clinical contexts:

Highlights of Our Services

• Advanced Technology: Utilizing cutting-edge technology for precise and reliable quantification of STAT5 activity.
• Comprehensive Analysis: Offering both phosphorylated and total STAT5 measurements for thorough assessment.
• Customizable Formats: Tailored assay formats to meet specific research objectives and experimental conditions.
• High-Throughput Capabilities: Capable of large-scale screenings to expedite the drug discovery process.
• Expertise and Experience: Over 20 years of industry experience, ensuring high-quality service and accurate data interpretation.

Frequently Asked Questions

Q1: How can this service benefit cancer research?

A1: By identifying the role of STAT5 in cancer progression and screening potential inhibitors or activators, our service aids in elucidating the molecular mechanisms of tumorigenesis and supports the development of targeted cancer therapies.

Q2: Can this service be customized for specific research needs?

A2: Absolutely. Creative Biolabs offers customizable assay formats tailored to meet the unique requirements of your research, providing flexibility and specificity in experimental design.

Creative Biolabs offers a comprehensive and high-quality assessment service for STAT5 activation and deactivation, leveraging advanced technologies and extensive expertise. Our services are tailored to support diverse research applications, driving progress in cancer research and immune-oncology.

Reference

1. Wingelhofer, Bettina, et al. "Implications of STAT3 and STAT5 signaling on gene regulation and chromatin remodeling in hematopoietic cancer." Leukemia 32.8 (2018): 1713-1726. Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only | Not For Clinical Use