SW-480 In Vitro RNA Expression Analysis Assay (Real-time PCR) (CAT#: ITS-1122-YF718)

SW-480

Human

SW480 [SW-480] cells isolated from the large intestine of a Dukes C colorectal cancer patient and can be used in cancer research.

SW480

Colorectal Adenocarcinoma; Colon Cancer

Oncology

In Vitro RNA Expression Analysis Assay (Real-time PCR)

SW-480-cell based In Vitro RNA Expression Analysis Assay (Real-time PCR)

Real-time PCR is the most commonly used platform to evaluate mRNA or miRNA expression after conversion of mRNA or miRNA into cDNA. Amplification of a target gene is evaluated by quantifying th fluorescence signal generated using DNA intercalating dyes (SYBER green) or gene-specific probes. This technique can also be used to screen gene mutations, DNA modifications and confirm results obtained from northern blotting or microarray. Additionally, real-time PCR is the most suitable tool for gene expression analysis for RNA samples with low yields compared to hybridization techniques.

Gene and Protein Expression Assays

Following transcription, primary transcript RNA is converted into mature RNA by post-transcriptional modifications. Post-transcriptional modification includes three major steps namely the addition of a 5'cap, addition of a 3'polyadenylation tail and RNA splicing. Stability of RNA has been considered as very important for gene expression and proteins at the post-transcriptional level.