Introduction

Spleen tyrosine kinase (SYK) is a pivotal non-receptor tyrosine kinase that plays significant roles in both adaptive and innate immune responses, cellular adhesion, osteoclast maturation, platelet activation, and vascular development. SYK is engaged in numerous pathologies including cancer and autoimmune diseases, making it a target of prime therapeutic interest. However, due to the complex nature of SYK's phosphorylation sites and interactions with other proteins, reliable assessment of its activation/deactivation states remains a challenging task. At Creative Biolabs, we provide cutting-edge services for the comprehensive evaluation of SYK kinase activity and its implications in various research fields.

Assessment of SYK Kinase Activation/Deactivation at Creative Biolabs

Biological Significance

SYK kinase is activated through the binding of its SRC homology 2 (SH2) domains to phosphorylated tyrosines in immunoreceptor tyrosine-based activation motifs (ITAMs)—a process that relieves SYK's autoinhibitory interactions and activates downstream signaling pathways. The kinase's role extends to mediating integrin signaling in various immune cells, participating in the innate immune response against pathogens via C-type lectins, and contributing to vascular development by assisting in the separation of newly formed lymphatic vessels from the blood vasculature.

Service Contents

Creative Biolabs offers a suite of technologically advanced solutions for assessing SYK kinase activation and deactivation:

• Total SYK Assay: A sandwich immunoassay designed for the quantitative detection of both phosphorylated and non-phosphorylated SYK.
• Peptide-Based Biosensors: Development and application of Syk-specific artificial peptide biosensors to detect intracellular SYK activity in live cells, maintaining physiological relevance.
• Phosphoprotein Detection: detect phosphorylated proteins in cell lysates post-stimulation, including p-SYK, SYK, and other related markers like P-AKT and P-PLCγ2.

Benefits for you

Application of Our Assessment of SYK Kinase Activation/Deactivation Services

• Cancer Research: Investigating SYK's role in oncogenesis, cell survival, and metastasis across different cancer types.
• Autoimmune and Inflammatory Diseases: Exploring SYK's involvement in diseases like rheumatoid arthritis and systemic lupus erythematosus.
• Drug Development: Screening potential SYK inhibitors and analyzing their efficacy in modulating kinase activity.
• Translational Medicine: Facilitating the translation of basic research findings to clinical diagnostics and therapeutic interventions.

Frequently Asked Questions (FAQs)

Q1: Can your assays be tailored for specific research needs?

A1: Yes, our services are highly versatile and can be customized to fit specific experimental designs and research requirements.

Q2: How do you ensure data accuracy and reproducibility?

A2: We employ stringent data normalization protocols, technical replicates, and thorough background subtraction to ensure the highest data quality.

Creative Biolabs is committed to advancing scientific understanding and therapeutic development targeting SYK kinase. Our comprehensive assessment services for SYK activation/deactivation provide researchers with the precise, physiologically relevant data necessary to drive innovations in cancer, autoimmune disease treatment, and beyond.

For Research Use Only | Not For Clinical Use