At Creative Biolabs, our team is committed to advancing the field of cancer research through our comprehensive bioassays for therapeutic antibody and protein development. Our A375-based proliferation assay service is meticulously designed to cater to the specific needs of the scientific community. Through these assays, researchers can evaluate the efficacy and potency of potential therapeutic candidates by investigating their impact on the proliferation rate of A375 cells. Our services ensure precise, reproducible, and high-throughput screening essential for groundbreaking cancer research.

A375 Cells for Melanoma Research

A375 is a human melanoma cell line that is widely used in cancer research. These cells were originally derived from a skin melanoma of a 54-year-old female patient and have since been adapted to grow continuously in culture. As a highly characteristic skin cancer cell line, A375 cells exhibit rapid growth and the ability to form tumors when introduced into animal models. This property makes them invaluable in understanding the mechanisms behind melanoma and testing the efficacy of novel therapeutic agents.

A375 Cell-based Proliferation Assay

Creative Biolabs provides several specialized methods to conduct A375 proliferation assays, ensuring the delivery of multifaceted data. Our methods include the use of cell viability assays, such as MTT, WST-1, and ATP-based luminescent readings, that measure the proliferative capacity of A375 cells. Additionally, we utilize advanced imaging techniques and flow cytometry for detailed cell cycle analysis. Upon completion of the assays, we provide comprehensive data sets that include dose-response curves and IC50 values, which are critical for understanding the concentration at which a substance inhibits 50% of the A375 cell proliferation. This detailed data supports researchers in identifying promising therapeutic candidates and optimizing treatment protocols.

Assay Protocol

One-Stop Platform for Diverse Cell Proliferation Assays

To support the accelerated development of cancer therapeutics, Creative Biolabs offers a one-stop platform for various cell proliferation assays. Beyond the A375-based proliferation assay services, our extensive portfolio includes proliferation assays based on a broad spectrum of other cancer cell lines, enabling cross-comparison and deeper insights into drug action. Equipped with cutting-edge technology and a team of experienced scientists, we are committed to delivering high-quality, reliable, and actionable data that accelerates the process of drug discovery and development. Please contact Creative Biolabs today to discuss your project needs and how we can support your scientific discoveries.

For Research Use Only | Not For Clinical Use