Creative Biolabs is a global leader in providing comprehensive, high-quality bioassay services tailored to the needs of clients in the fields of therapeutic antibody and protein development. Our extensive repertoire of bioassays, which includes cell proliferation assays, is designed to support the various stages of drug discovery and development.

A431 Cells for Melanoma Research

The A431 cell line, derived from human epidermoid carcinoma, is extensively utilized in cancer research due to its unique properties. These cells are known for their overexpression of the epidermal growth factor receptor (EGFR), making them an invaluable model for studying EGFR-related pathways and their implications in cancer biology. By leveraging this cell line, researchers can gain crucial insights into tumor biology, drug resistance mechanisms, and therapeutic efficacy. A431 cells are a valuable tool for testing the efficacy and safety of compounds, specifically those targeting the EGFR pathway. Thus, the A431 cell line plays an important role in the development of targeted cancer therapies, providing a robust and reliable system for bioassays.

A431 Cell-based Proliferation Assay Service

At Creative Biolabs, our A431-based proliferation assay services are tailored to meet the exact needs of research and development projects. Utilizing a variety of well-established methods, we ensure comprehensive analysis and reliable data outcomes. Upon completion of the assays, we deliver comprehensive data packages including the original experimental data, detailed analysis results, the complete experimental workflow, and a full report. We offer data analysis results including dose-response curves and IC50 values, enabling researchers to assess the efficacy and potency of tested compounds comprehensively.

Diverse Cell Proliferation Assay Techniques

The methods we employ for A431-based proliferation assays include:

• Colorimetric Assays

Colorimetric assays rely on the color change that occurs in response to enzymatic activity or the presence of a particular compound, which correlates with cell proliferation.

Examples: MTT, XTT, MTS, WST-1

• Label-based DNA Synthesis Assays

These assays involve the incorporation of radioactive nucleotides into newly synthesized DNA, providing a direct measure of DNA synthesis and proliferation.

Examples: BrdU incorporation assay

• Fluorescence-Based Assays

These assays use fluorescent dyes or probes to measure cell proliferation directly or indirectly.

Examples: Alamar Blue, CFSE

• Proliferation Marker Assays

These assays utilize flow cytometry to detect specific proliferation markers expressed during cell division.

Examples: Ki-67, PCNA

• ATP Assay

ATP is an indicator of metabolically active cells. This method measures cellular ATP levels, which correlate with viable cell numbers.

Advantages of Our Services

• High Sensitivity and Specificity: Utilizing advanced techniques to ensure accurate and reproducible results.
• Expertise and Experience: A dedicated team of scientists with extensive experience in cell proliferation assays.
• Customized Solutions: Tailored assay designs to meet the unique requirements of each project.
• Fast Turnaround Time: Efficient processes to deliver results promptly.

Partner with us for your A431-based proliferation assay needs and benefit from our commitment to excellence. For more information on our services, please contact Creative Biolabs.

For Research Use Only | Not For Clinical Use