The humanized antibodies are a type of antibodies derived from non-human species, whose protein sequences have been modified to improve their similarity with human natural antibody variants. Currently, antibody humanization is acknowledged to play a fundamental role in the remarkable progress of antibodies as therapeutic agents.
Fig.1 Humanized antibody. (Jones, 1986)
Recombinant antibody technology is rapidly becoming available and displaying considerable success in clinical practices. However, the immunogenicity of murine monoclonal antibodies is observed to be restrictive in cancer immunotherapy. To reduce the chance of immunogenicity, humanized antibodies are created to address immunogenic response and thus they are considered to be a promising alternative reagent in the clinic. In Creative Biolabs, we possess a variety of methods and toolkits used in the antibody humanization process and provide customized antibody humanization services.
Creative Biolabs has launched the GHA® Platform to develop humanized antibodies as well as Hi-Affi™ Platform to develop human monoclonal antibodies from non-human primates. The steps for designing humanized antibodies are as follows.
Fig.2 A humanized process in Creative Biolabs.
i. Universal antibody humanization service or one-stop, customized antibody service for special needs.
ii. 30 days guaranteed services for the whole project, once clients’ VH and VK sequences received.
iii. High-quality service. We deliver humanized candidates and ensure their epitope specificity and binding affinity.
iv. If clients are satisfied with humanized antibody variants, we can provide the stable cell line further optimized for high yield expression and validated for scale-up.
Fig.3 Diverse antibody forms. (Carter, 2001)
✔ Rich Experience: Creative Biolabs has extensive experience in antibody engineering for diagnostics and therapeutics and there are more than 15 murine humanization projects have been accomplished successfully.
✔ Strategy Diversity: We have multiple toolkits for humanized antibodies, including CDR back mutation library screening, human framework selection, etc.
✔ Species Diversity: Our humanization services to antibodies can be allowed to any species, such as non-human primate (NHP), rabbit, llama, dog, chicken, etc.
✔ Guaranteed Quality: The optimized antibody with minimal immunogenicity, almost equivalent to a mature human antibody, is guaranteed to remain parental affinity and specificity. The affinity maturation can improve the affinity by at least 10 folds.
✔ Free of Rights: Clients would get the full ownership of humanized antibodies from our customized platform.
✔ Full Suit of Characterization Assays: Binding assays by ELISA (Enzyme-linked immuno sorbent assay), thermostability, aggregation rate, KD determination against soluble antigens, IC50 (Half maximal inhibitory concentration), SPR (Surface plasmon resonance), endotoxin detection, etc.
With the flourish of antibody drugs, the demand for humanized antibodies is rapidly growing. Due to strict ethical and moral principles, it is totally impossible to immunize humans directly with interested antigens. Here, Creative Biolabs sets up two reliable platforms for humanized antibodies with high-affinity, low immunogenicity and has extensive experience in providing multiple diverse antibody humanization services for both laboratory and therapeutic purposes. If there is any question, please contact us for more information.
For Research Use Only | Not For Clinical Use