Highly sensitive sandwich ELISA for quantitative detection of mouse ALPL in serum, plasma, and tissue samples.
Brand
BiliTrack™
Development Stage
ADME
ADME Research Domain
Excretion
Description
BiliTrack™ High-Sensitivity Mouse Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ELISA Kit is a sandwich immunoassay designed for ultra-sensitive quantification of ALPL in mouse serum, plasma, and tissue lysates. It enables precise measurement of osteoblast activity and bone metabolism biomarkers in preclinical research, supporting skeletal disease and drug development studies.
Features
Ultra-sensitive sandwich ELISA format enables accurate detection of low-abundance mouse ALPL in diverse biological matrices including serum and tissues samples. High specificity antibodies reduce cross-reactivity, ensuring reliable quantification of ALPL with minimal background interference across experimental conditions consistently reproducible results. Pre-coated microplate and ready-to-use reagents streamline workflow reduce variability and improve reproducibility in high-throughput research laboratories ELISA assay performance enhanced. Optimized sensitivity supports early detection of ALPL changes associated with bone formation, remodeling, and metabolic disease progression studies research applications.
Process Relevance
Enables sensitive monitoring of ALPL expression to assess bone formation dynamics and metabolic activity in experimental models.
Application Stage
Preclinical research, bone biology studies, drug discovery, and translational skeletal disease model evaluation.
Applications
ALPL quantification in mouse serum, plasma, and tissues; bone formation studies; osteoblast differentiation assays; skeletal disease models; ADME profiling studies; biomarker validation; and pharmacological intervention research studies applications.
Serum, plasma, tissue homogenates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Bone metabolism, osteogenesis, enzymology, skeletal disease research, pharmacokinetics (ADME), preclinical pharmacology, etc.
Size
96 tests
Data Output Type
Quantitative colorimetric measurement of mouse ALPL concentration using sandwich ELISA.
Assay Range
0.156 ng/ml - 10 ng/ml
LOD
< 0.07 ng/ml
Species
Mouse
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
ALPL is an essential osteogenic enzyme involved in mineralization processes during bone development and remodeling. Its expression reflects osteoblast differentiation status and is widely used as a biomarker in skeletal research.
Pathways
ALPL participates in phosphate metabolism and extracellular matrix mineralization pathways essential for hydroxyapatite formation. It is regulated downstream of RUNX2-driven osteogenic signaling and linked to osteoblast maturation processes.