BiliTrack™ Low Sample Volume Human Alkaline Phosphatase (ALP) ELISA Kit

CAT#: ITS-0326-WB138
Product Type: ELISA Kit
Species: Human
Target: Human tissue non-specific and tissue-specific ALP isoforms
Brand: BiliTrack™
Development Stage: ADME
ADME Research Domain: Excretion
Short Description
Quantitatively measures human ALP levels in low-volume biological samples with high sensitivity.
Brand
BiliTrack™
Development Stage
ADME
ADME Research Domain
Excretion
Description
BiliTrack™ Low Sample Volume Human ALP ELISA Kit enables precise in vitro quantification of ALP in tissue homogenates, cell lysates, culture supernatants, and other biological fluids. Designed for low-volume samples, it ensures accurate, reproducible detection across a wide dynamic range.
Features
Sensitive colorimetric sandwich ELISA detecting ALP in concentrations as low as 0.56 ng/mL, ensuring high-resolution quantification of biological samples.
Optimized for low sample volumes, allowing robust measurement from limited tissue, cell lysates, or biological fluids with minimal material.
Wide dynamic range from 1.56 ng/mL to 100 ng/mL, enabling detection of both baseline and elevated ALP activity in diverse studies.
Stable lyophilized standards with consistent performance, minimal activity loss (<5%) over 12 months under recommended storage conditions.
Process Relevance
Monitors ALP activity to support metabolic profiling and disease biomarker validation in low-volume clinical or preclinical samples.
Application Stage
Drug discovery, preclinical research, biomarker validation, and in vitro experimental studies requiring limited sample volume.
Applications
Suitable for measuring ALP in serum, plasma, cell culture supernatants, tissue homogenates; supports biomarker studies, ADME profiling studies, and enzymatic activity profiling in research.
Target
Human tissue non-specific and tissue-specific ALP isoforms
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
Assay Measures
Quantitative
Research Areas
Bone metabolism, liver function assessment, hepatology, endocrinology, pharmacokinetics (ADME), metabolic disease research, pharmacology, clinical diagnostics, etc.
Size
96 tests
Data Output Type
Quantitative enzyme activity measurement using endpoint colorimetric detection.
Assay Range
1.56 ng/ml - 100 ng/ml
LOD
< 0.56 ng/ml
Species
Human
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Alkaline phosphatase is a hydrolase enzyme involved in phosphate metabolism and cellular differentiation. Altered ALP levels are key biomarkers in liver, bone, and metabolic disorders, informing disease progression and therapeutic interventions.
Pathways
ALP participates in phosphate hydrolysis and mineralization pathways, interacting with bone morphogenetic and hepatocyte metabolic signaling. Dysregulation may affect skeletal development and hepatic function downstream.
For Research Use Only | Not For Clinical Use
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