BiliTrack™ Standard Mouse Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ELISA Kit

CAT#: ITS-0326-WB155
Product Type: ELISA Kit
Species: Mouse
Target: Mouse tissue-nonspecific alkaline phosphatase (ALPL) enzyme
Brand: BiliTrack™
Development Stage: ADME
ADME Research Domain: Excretion
Short Description
Quantifies mouse ALPL levels in biological samples using a standard sandwich ELISA method.
Brand
BiliTrack™
Development Stage
ADME
ADME Research Domain
Excretion
Description
BiliTrack™ Standard Mouse Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ELISA Kit is a reliable immunoassay designed for accurate measurement of ALPL in mouse samples. Based on a sandwich ELISA format, it enables consistent detection in serum, plasma, and tissue lysates, supporting research in bone metabolism, osteogenesis, and skeletal disease studies.
Features
Standard sandwich ELISA ensures accurate detection of mouse ALPL with strong specificity and minimal cross-reactivity in biological samples.
Optimized antibody system provides stable performance and reproducible quantification across multiple experimental batches.
Pre-coated microplate and ready-to-use reagents streamline workflow and reduce variability in assay execution.
Compatible with serum, plasma, and tissue lysates, supporting flexible application in routine preclinical research settings.
Process Relevance
Enables routine assessment of ALPL expression to monitor bone formation and metabolic enzyme activity.
Application Stage
Basic research, preclinical evaluation, and routine laboratory analysis.
Applications
Quantification of ALPL in mouse serum, plasma, and tissues; bone metabolism studies; osteogenesis research; enzyme activity measurement; ADME profiling studies; biomarker analysis in skeletal disease models.
Target
Mouse tissue-nonspecific alkaline phosphatase (ALPL) enzyme
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Bone metabolism, osteogenesis, enzymology, skeletal disease research, pharmacokinetics (ADME), preclinical pharmacology, etc.
Size
96 tests
Data Output Type
Standard quantitative measurement of ALPL protein concentration via colorimetric ELISA detection.
Assay Range
0.16 ng/ml - 10 ng/ml
LOD
0.09 ng/ml
Species
Mouse
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Alkaline phosphatase (ALPL) is a key enzyme involved in dephosphorylation processes essential for bone formation and skeletal development. In mice, it is widely used as a biomarker of osteoblast activity. Altered ALPL expression reflects changes in bone turnover and skeletal health status.
Pathways
ALPL functions in phosphate metabolism and biomineralization pathways, promoting hydroxyapatite deposition in bone tissue. It is regulated downstream of osteogenic transcription factors such as RUNX2. Its activity reflects osteoblast differentiation and systemic mineral homeostasis regulation.
For Research Use Only | Not For Clinical Use
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