BiliTrack™ Rabbit Alkaline Phosphatase, Tissue-nonspecific (ALPL) ELISA Kit

CAT#: ITS-0326-WB148
Product Type: ELISA Kit
Species: Rabbit
Target: Detects rabbit tissue-nonspecific alkaline phosphatase (ALPL) protein
Brand: BiliTrack™
Development Stage: ADME
ADME Research Domain: Excretion
Short Description
Quantitative detection of rabbit ALPL levels in biological samples via sandwich ELISA.
Brand
BiliTrack™
Development Stage
ADME
ADME Research Domain
Excretion
Description
BiliTrack™ Rabbit Alkaline Phosphatase, Tissue-nonspecific (ALPL) ELISA Kit enables sensitive and specific quantification of ALPL in rabbit-derived samples. Utilizing a robust sandwich ELISA format, this kit supports reliable detection across serum, plasma, and tissue lysates, facilitating research in enzyme regulation, bone metabolism, and hepatobiliary function.
Features
High-sensitivity sandwich ELISA design ensures accurate quantification of low-abundance ALPL in complex biological matrices.
Pre-coated microplate and optimized reagents streamline workflow and improve inter-assay reproducibility.
Broad sample compatibility including serum, plasma, and tissue lysates enhances experimental flexibility.
Validated protocol with stable standards supports consistent performance across multiple experimental conditions.
Process Relevance
Quantifies ALPL to evaluate enzymatic activity changes in metabolic and tissue-specific biological processes.
Application Stage
Preclinical research, biomarker validation, and enzymatic pathway analysis.
Applications
Measurement of ALPL in rabbit serum, plasma, and tissues; enzyme activity studies; ADME profiling studies; bone and liver research; biomarker quantification; validation of metabolic and pathological models.
Target
Detects rabbit tissue-nonspecific alkaline phosphatase (ALPL) protein
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Bone metabolism, hepatology, enzymology, metabolic disease research, pharmacokinetics (ADME), biomarker discovery, etc.
Size
96 tests
Data Output Type
Quantitative protein concentration measurement via colorimetric ELISA readout.
Assay Range
0.312 ng/ml - 20 ng/ml
LOD
< 0.115 ng/ml
Species
Rabbit
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Alkaline phosphatase (ALPL) is a membrane-bound enzyme involved in dephosphorylation processes critical for mineralization and metabolic regulation. It is widely expressed in liver, bone, and kidney tissues. Altered ALPL levels are associated with bone disorders, hepatic dysfunction, and systemic metabolic imbalances.
Pathways
ALPL participates in phosphate metabolism and is closely linked to pathways regulating biomineralization and extracellular matrix formation. It acts downstream of osteoblast differentiation signals and influences hydroxyapatite deposition. Additionally, ALPL activity reflects hepatobiliary transport and metabolic homeostasis processes.
For Research Use Only | Not For Clinical Use
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