BiliTrack™ High-Sensitivity Rat Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ELISA Kit

CAT#: ITS-0326-WB153
Product Type: ELISA Kit
Species: Rat
Target: Rat tissue-nonspecific alkaline phosphatase (ALPL) enzyme
Brand: BiliTrack™
Development Stage: ADME
ADME Research Domain: Excretion
Short Description
Ultra-sensitive quantification of rat ALPL levels in biological samples using sandwich ELISA.
Brand
BiliTrack™
Development Stage
ADME
ADME Research Domain
Excretion
Description
BiliTrack™ High-Sensitivity Rat Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ELISA Kit is a highly sensitive immunoassay designed for precise quantification of ALPL in rat samples. Based on a sandwich ELISA format, it enables reliable detection in serum, plasma, and tissue lysates, supporting bone metabolism, osteogenesis research, and preclinical disease modeling.
Features
High-sensitivity sandwich ELISA enables accurate detection of low-abundance rat ALPL in complex biological matrices with strong analytical performance.
Highly specific antibody pairing ensures selective recognition of tissue-nonspecific ALPL with minimal cross-reactivity in experimental samples.
Pre-coated microplate and optimized reagents streamline experimental workflow and ensure high inter-assay reproducibility and consistency.
Compatible with serum, plasma, and tissue lysates, providing flexible application across diverse preclinical research models.
Process Relevance
Enables sensitive monitoring of ALPL activity changes during bone formation and metabolic regulation processes.
Application Stage
Preclinical research, drug evaluation, and bone metabolism model studies.
Applications
Quantification of ALPL in rat serum, plasma, and tissues; bone formation studies; osteoporosis research; ADME profiling; enzyme activity profiling; preclinical biomarker analysis in disease models.
Target
Rat tissue-nonspecific alkaline phosphatase (ALPL) enzyme
Detection Method
Colorimetric
Sample Type
Serum, plasma and other biological fluids.
Assay Measures
Quantitative
Research Areas
Bone metabolism, osteogenesis research, enzymology, metabolic disease research, pharmacokinetics (ADME), preclinical pharmacology, etc.
Size
96 tests
Data Output Type
High-sensitivity quantitative measurement of ALPL protein concentration via colorimetric ELISA detection.
Assay Range
0.63 ng/ml - 40 ng/ml
LOD
0.38 ng/ml
Species
Rat
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Alkaline phosphatase (ALPL) is a critical enzyme involved in dephosphorylation processes essential for bone mineralization and skeletal development. In rats, it serves as a key biomarker for osteoblast activity and bone formation dynamics. Altered ALPL levels are closely associated with metabolic bone disorders.
Pathways
ALPL functions within phosphate metabolism and bone biomineralization pathways, promoting hydroxyapatite crystal formation. It acts downstream of osteogenic differentiation signals such as RUNX2-regulated pathways. Its enzymatic activity directly reflects osteoblast function and systemic mineral homeostasis regulation.
For Research Use Only | Not For Clinical Use
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