BiliTrack™ Standard Rat Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ELISA Kit

CAT#: ITS-0326-WB154
Product Type: ELISA Kit
Species: Rat
Target: Rat tissue-nonspecific alkaline phosphatase (ALPL) enzyme
Brand: BiliTrack™
Development Stage: ADME
ADME Research Domain: Excretion
Short Description
Quantifies rat ALPL levels in biological samples using a validated sandwich ELISA method.
Brand
BiliTrack™
Development Stage
ADME
ADME Research Domain
Excretion
Description
BiliTrack™ Standard Rat Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) ELISA Kit is a robust immunoassay designed for accurate measurement of ALPL in rat samples. Utilizing a sandwich ELISA format, it enables reliable detection in serum, plasma, and tissue lysates, supporting research in bone metabolism, osteogenesis, and preclinical disease modeling.
Features
High-specificity sandwich ELISA enables accurate detection of rat ALPL with minimal background interference in complex biological matrices.
Optimized antibody pairing ensures stable binding performance and reproducible quantitative results across experimental runs.
Pre-coated microplate system and standardized reagents simplify workflow and reduce technical variability between assays.
Compatible with multiple rat-derived sample types, supporting flexible application in diverse preclinical research settings.
Process Relevance
Enables consistent monitoring of ALPL expression to evaluate bone formation and metabolic enzyme activity.
Application Stage
Preclinical research, experimental modeling, and routine biochemical analysis.
Applications
Quantitative detection of ALPL in rat serum, plasma, and tissues; bone formation studies; osteogenesis analysis; enzymatic activity assays; ADME profiling studies; biomarker evaluation in disease models.
Target
Rat tissue-nonspecific alkaline phosphatase (ALPL) enzyme
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
Assay Measures
Quantitative
Research Areas
Bone metabolism, osteogenesis, enzymology, metabolic disease research, pharmacokinetics (ADME), preclinical pharmacology, etc.
Size
96 tests
Data Output Type
Quantitative measurement of ALPL protein concentration via colorimetric ELISA detection.
Assay Range
3.12 ng/ml - 200 ng/ml
LOD
< 1.15 ng/ml
Species
Rat
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Alkaline phosphatase (ALPL) is a key enzyme involved in dephosphorylation processes essential for skeletal development and mineralization. In rats, it is widely used as a biomarker of osteoblast activity and bone formation. Dysregulation of ALPL reflects altered bone turnover and metabolic imbalance.
Pathways
ALPL participates in phosphate metabolism and biomineralization pathways, promoting hydroxyapatite deposition in bone tissue. It acts downstream of osteogenic differentiation signaling pathways such as RUNX2-mediated transcription. Its activity directly reflects osteoblast function and systemic mineral homeostasis.
For Research Use Only | Not For Clinical Use
Online Inquiry